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Questions related to Cancer Stem Cells
I am trying to characterize normal colon stem from the remaining stromal cells and other cells found in the normal colon. I want to know if injecting normal colon stem cells into athymic mice can...
04 April 2018 589 0 View
From A549 and H1299 cells, After isolation which media can be used to maintain cancer stem cells?
30 March 2018 1,256 6 View
We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...
21 January 2018 7,874 2 View
I transfected GipZ lentiviral shRNA with packaging plasmids psPAX2 and VSVG into 293T cells. I also transfected LentiCRISPRv2GFP (lentiviral based GFP containing CRISPR from Adgene). The...
16 January 2018 6,355 2 View
01 January 2018 4,955 2 View
I am studying vasculogenic potential of cancer stem cells, and so I culture stem cell spheres on Matrigel. After several days, they form 3D tube structures into the gel. Inhibition of my protein...
29 November 2017 9,014 8 View
we receive tissue at 5 pm for cancer stem cell estimation 2nd day 10 am , is it better to leave the tissue at 4 C or -20C ?
17 November 2017 2,990 1 View
It is quite known that it requires up to 24 hours exposures in hypoxic condition (1% O2) to establish acute hypoxia in cancer cells cultured in monolayer, whereas > 24 hours (2-5 days) have...
11 November 2017 6,137 2 View
I wish to maintain my sorted leukemic stem cells as "stem cells" and keep them from differentiating. Kindly suggest some ways in which this can be done. (Except for media without serum).
09 September 2017 9,810 3 View
I am trying to isolate cancer stem cells from MCF-7 and MDA MB231 with the help of tumorsphere assay. Is sorting necessary before tumorsphere assay cell seeding or do we sort cells after assay?...
09 September 2017 5,232 6 View
I want to know about situation that cancer stem cell remain stemness and undifferentiated?
09 September 2017 6,221 2 View
08 September 2017 2,846 3 View
I would like to know your experience on culturing Human breast cancer stem cells (Parental) Thanks.
11 August 2017 5,039 3 View
I tested an extract on cancer stem cells , some times has good effect ( 60% ) some times 2% on another tissue, and some times has no effect ? where is the fault ? OR/ tissues are differently...
08 August 2017 9,021 3 View
Could anyone provide me some information regarding markers specific for oral cancer stem cells?
08 August 2017 5,868 7 View
08 August 2017 7,296 4 View
Dear Ladies and Gentlemen, I tried some herbal extracts on Cancer stem cells but no response till 6 mg. as a single dose ,and the result was increasing the number of caner stem cells , is it...
08 August 2017 2,157 1 View
01 August 2017 9,984 3 View
I want to detect the somatic mutations for breast cancer disease. Can anyone tell me about the major differences in detecting somatic mutations from blood sample and tissue samples
21 July 2017 10,088 3 View
07 July 2017 3,077 4 View
Hi, I am using "EasySep Direct Human CTC Enrichment Kit" from STEMCELL Tech. for CTC isolation. Is anyone using it? I am also planning to check EpCAM, CK8 and CD45 by PCR for its primary...
29 June 2017 7,096 3 View
06 June 2017 5,068 4 View
Hi, everyone ! I am going to do qPCR to determine the expression level of 3 germ layer marker gene, I noticed in some papers Gata4, Gata6 are employed as endoderm markers; T, Tbx5, Flk1 are...
06 June 2017 4,572 5 View
If the Cancer Stem Cell is the mutated form of stem cell,the DNA in both is different.
25 May 2017 396 2 View