Why most of the studies use cisplatin i.p injection at dose 3 mg/kg?

I will start a cisplatin treatment soon for NU/NU Nude Mice (Crl:NU-Foxn1nu) and I found multiple publications using 3 mg/kg i.p injection with nude mice. There were different injection frequency...

11 December 2018 5,028 2 View

In Springer publications, my article has two status for two weeks now without any further contacts from them?

My article in Medical Oncology is in two places "submission with decision" which is accept and in "submission with production complete". We didn't get any mails from them though. Any one had the...

06 July 2018 4,886 0 View

Why serum free conditions are used to isolate cancer stem cells?

I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?

05 June 2018 7,182 4 View

What is the difference between stemness and self-renewal?

In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?

04 May 2018 9,544 4 View

Anyone has a protocol for chondrogenic differentiation of MSCs in monolayer ? and a method of quantification of the results?

I am trying to avoid the pellet protocol

04 May 2017 7,481 0 View

In sphere forming test, should i change the medium during the test period? and if yes how to do that in low attachment plates?

09 October 2016 10,022 0 View

I need to do the Transwell migration and invasion assay on squamous cell carcinoma cell line. anyone know the protocol and where can i get the plates?

working on SCC12 cell line where i can get the Matrigel?

07 August 2016 1,023 2 View

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)?

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)? need to get 5 fluorouracil as a chemotherapy for SCC

06 July 2016 5,772 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View