hello,
Is there anyone who has sorted MCF-7 using CD24-/ CD44++/CD326++(epcam) markers. I got articles with CD24/44. Please share some articles with FACS profile from mcf-7 and MDAMB 231 with these markers.
Hello, everyone, I need plasmid for Lentiviral formation. Is there any lab in India which is using Lentiviral packaging plasmid. psPAX2 and pMD2.G. Please let us know.
08 September 2017 8,941 0 View
We are doing LDHA assy for mammalian cells. we are using conc enzyme .15 to .5 u/ml, pyruvate 2mM or NADH 20microM, for the standard curve but we are getting almost same absorbance. Please...
08 September 2017 6,162 0 View
I am trying to isolate cancer stem cells from MCF-7 and MDA MB231 with the help of tumorsphere assay. Is sorting necessary before tumorsphere assay cell seeding or do we sort cells after assay?...
08 September 2017 5,268 6 View
We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...
08 September 2017 4,961 7 View
We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...
08 September 2017 2,943 5 View
I want to do tumorsphere assay with MDAMB231 it is usually grown in L-15 or RMI 1640 media. So, do I need to prepare cancer stem cell CSCs specific media in L-15 or can I use CSCs media prepared...
07 August 2017 8,207 3 View
We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...
06 July 2017 10,099 3 View
is there a major difference between sodium phosphate buffer and potassium phosphate buffer. can they be used alternatively?
06 July 2017 9,031 4 View
Hello, I want to study knockdown of a gene in cell. we are planing to buy it from sigma I have chosen the vector with shRNA insert but I am not able to understand how to choose scramble for this...
04 May 2017 8,429 1 View
Hello, I have isolated cancer stem cells from MCF-7 cells. I want to characterize these cells. Is there any option available other than FACS? Can we do it with PCR or western blot for markers of CSCs?
03 April 2017 427 5 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View