Hey Diem,

You may try fluorescence method using an activatable agent, MMP sense 680 probe, the shortcoming is the specificity of this probe because this probe may be activated by several MMPs, such as MMP-2, -3, -9 and -13. But it is very sensitive.

http://www.perkinelmer.com/catalog/product/id/nev10126

Good luck

Hi Diem,

You can use label-free biosensors that can handle crude samples, e.g. Attana.

You can immobilize antibody and inject cell culture supernatant, plasma or serum

Compare to ELISA kit already available (http://www.rndsystems.com/Products/dmp900/), i guess it will be faster once set it up with the Attana plus different antibodies can be tested, since different form of MMP-9 are produced by the cells, it offers more flexibility on the experiment and the possibility to get more detailed results, such as kinetics and specificity, then a commercial ELISA. Given that you you say you are developing a new biosensors for this I think added information will be important for you.

Feel free to contact me if you have questions.

Good luck!

Teodor

OK, fluorescence method using MMP sense 680 probe. Also, there are some fluorescent probe which can bind to the MMP in the secreted media which can be detected by SDS-PAGE gel and scanning the gel itself. This is the best i worked with these probes on protease. But i got this as a gift sample probe from the Inventor scientist.

Thank you so much for your help, I'm very appreciated it!