Is it normal to get higher cell activity in test during Cytotoxicity assay

I used CCk-8 kit (works on dehydogenase activity in viable cells) to do cytoxicity assay and I got higher absorbance (@450 nm, reference- 650 nm) for my tests than control (cells in microwell...

03 April 2016 8,631 2 View

For MTT assay, how do you select cell line?

11 December 2015 6,289 2 View

Difference between silk produced by spider and silkworm?

regards Jitendra

11 December 2014 9,589 0 View

What are the merits/demerits in using S-nitroso human serum albumin for cancer treatment?

Thanks and regards Jitendra

11 December 2014 5,079 5 View

Why bacterial system is still used for protein production?

Why bacterial system is still used for protein production for some protein knowing that it doesn't have a system to support post translational modification?

11 December 2014 5,971 5 View

What could be the reason of getting a lawn of transformed cells on LB plate?

10 November 2014 4,636 4 View

What is the pI of a monoclonal antibody?

I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).

10 November 2014 4,700 3 View

What happens to the enzyme kinetics when in solution and when immobilized?

10 November 2014 3,400 15 View

Why is it important to know the protein conc. accurately to know the protein structure using circular dichroism?

Thanks

09 October 2014 2,142 10 View

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield?

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield? Suggestions can cover centrifugation, changing the concentration of...

08 September 2014 9,297 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View