In my experiment, I collected condition media from breast cancer cell culture MDA-231 and I added it to normal breast cell to convert it to cancer, what is the advantage when we used condition media?
And what is better, should I study the MDA-231 cell or I study cell after dealing with condition media?
Condition media is used to show that spefcific cell type produces certain mediators that can affect cell phenotype. It can be the first step for finding out the factor that really control the phenotypical change. But, it can be growth factor, miRNA, biological molecules, or anything.
Condition media is used to show that spefcific cell type produces certain mediators that can affect cell phenotype. It can be the first step for finding out the factor that really control the phenotypical change. But, it can be growth factor, miRNA, biological molecules, or anything.
Here is everything you need to know.
MATER METHODS 2013;3:175
Cell Culture Media: A Review
last modified : 2014-08-25; original version : 2013-03-05
Abstract
A comprehensive review of cell culture media and Labome survey results on cell culture media from 720 formal publications.
Conditioned medium from cancer cell will not transform normal breast cancer cell to cancer cell. It might induce some morphological changes due to presence of secreted cytokines and proteins from cancer cells. What is your aim of the experiment and what exactly u want to study?
my aim is to study the gene LKB1 in fibroblastic breast after dealing with condition media of breast cancer
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