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Questions related to Cancer Cell Line
I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...
03 July 2019 6,073 2 View
We are attempting to induce hypoxic reponse in various breast cancer cells lines with the use of CoCl2 (more specifically using stock of 0.1M CoCl2 that was ordered already prepared and adding...
17 June 2019 5,493 3 View
Hi, Is there a good database for immortalized human cell lines? In particular cancer cell lines? Many times I need to look up attributes of a cell line; size, protein expression, doubling time,...
06 June 2019 8,544 6 View
We have isolated the exosome and preserve it at -20 degree freezer for about one month. Is the sample still suitable for morphology check under transmission electron microscopy or we must need to...
29 May 2019 9,450 5 View
Hi everybody, I want to start colony formation assay to test the potential citotoxicity of a mixture of compounds. I would like to test this mixture on 8505c cells by using this protocol:...
28 May 2019 4,695 3 View
We have tried conducting tube formation assay for RF/6A (Choroid Retinal Endothelial Cells obtained from Rhesus species). Passage number is 46. We have used MEM (ATCC recommended) medium...
27 May 2019 2,189 0 View
What would be right (to be ideal or Potential) concentration (ug/ml) of a plant crude drug extract at 50% inhibition (IC50) values against MCF-7 human breast cancer cell lines? I have red an...
15 May 2019 4,746 3 View
Dear all, I'm new in RNA extraction, cDNA synthesis and RT-PCR. I have isolated RNA from 20 samples of Sorghum by Triazol and continued with SV total kit (Promega), then measured on Nanodrop. the...
14 May 2019 8,648 3 View
I did some SPR experiments in another lab, but now that I am back in my own lab we do not have a biacore instrument and thus neither the software. Can anyone please recommend a software to just...
14 May 2019 1,923 6 View
Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...
05 May 2019 1,859 3 View
05 May 2019 6,830 3 View
Hi, I have been doing flow cytometry for cell cycle analysis using PI for a liver cancer cell line and I have been getting a big and strange peak where S-phase should be. Not sure if it's S-phase...
30 April 2019 776 7 View
I search a lot to find the protocol assay for measurment of enzyme activity of glutaminase but i can not find the best method.please help me.
12 April 2019 3,154 2 View
04 April 2019 1,200 8 View
Can someone give the brief of siRNA knock down procedure of a gene? How does it work? I want to check the role of one of the protein in some of the breast cancer cell line. For this ist i am gonna...
04 April 2019 2,059 6 View
04 April 2019 5,864 2 View
Hello. I am looking for any resource like a internet platform or something like that where I can see the main metabolism of the principal cancer cell lines. Thanks in advance.
04 April 2019 7,096 3 View
I was wondering if anybody knows what is the half-life of 4(OH)TAM in vitro? I am adding 4(OH)TAM at 0.5ng/mL in my murine ESC cultures every 24 hours for 5-6 days and I was wondering if this is...
28 March 2019 7,977 2 View
Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...
24 March 2019 10,018 3 View
As I conduct the Annexin V & 7-AAD staining experiment for cancer cell lines such as PC3, but I cannot decide the time when the positive control namely 5-fu would work? And could anyone know...
07 March 2019 9,553 1 View
03 March 2019 1,909 1 View
I want to perform assays with cancer cell lines during the next weeks and I will grow my cells with and without FCS, afterwards I want to test different growth enhancers and growth inhibitors. Do...
03 March 2019 792 2 View
03 March 2019 2,126 3 View
Hi, I am working with oesophageal cells and treating them to induce stemness. I would like to test for proliferation and colony forming ability. Would a soft agar assay or matrigel be better to...
03 March 2019 3,425 0 View