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Questions related to Cancer Cell Line
I am culturing breast cancer cell line, some time I observe high density of tiny dot like structure, I am not able to find out whether its contamination or cell debris.if it is contamination then...
02 February 2019 7,043 1 View
I am beginner in mammalian cell culture, I am maintaining breast cancer cell line (t47d,mdamb231,mdamb468,mcf7). Cells were going well but suddenly i observed so much cell debris and slightly...
02 February 2019 9,866 4 View
Can anyone help: what is best way to prove or show the relationship between Caspase 7 induction and cell signalling pathway or other cellular proteins in PTEN knocked-out colorectal cancer cell...
02 February 2019 8,376 2 View
I am looking for any good/detailed protocols on developing drug-resistant cancer cell lines? Thanks in advance.
02 February 2019 2,355 2 View
By using plant extracts s pellet , how could I know its concentration and how much DMSO it needs to dissolve it in ?
28 January 2019 3,403 3 View
Hi all, I am planning to treat few cancer cell lines with recombinant IFN-g in-vitro. I am curious to know from researchers with prior experience if one needs to be careful of the IFN-g levels...
23 January 2019 5,803 2 View
I am planning to DNA promoter methylation in breast cancer tissue, formalin fixed paraffin embedded tissue and breast cancer cell lines. Which bisulfite modification kit will be most effective...
21 January 2019 8,616 2 View
I was trying to perform imaging with mixed glial cells in L-15 media. The microscope had some issue so I couldn't image them on time. I was wondering whether I can still image the cells in L-15...
16 January 2019 9,557 0 View
How it will work for adherent cells and cells in suspension? Is there any cells toxicity probability?
11 January 2019 1,181 3 View
I'm going to use G6PDH as cytoplasm marker but I haven't found any information about a good nucleus marker in YEASTS. Thanks!
09 January 2019 3,347 1 View
I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...
04 January 2019 1,816 3 View
bioinformatics
01 January 2019 9,475 0 View
01 January 2019 5,963 2 View
Dear all, I have some compounds which good activity against some cancer cell lines (such as DU145 (Human prostate Cancer), MDA MB-231 (human breast cancer), etc. Now I want to search for the...
01 January 2019 8,171 7 View
01 January 2019 9,882 4 View
01 January 2019 5,105 2 View
These three breast cancer cell lines are all germline mutations of BRCA1 gene?
01 January 2019 4,460 3 View
Hello, I am looking for a TF activation array to test human breast cancer cell lines? What about this "TF Activation Profiling Plate Array II Catalog Number FA-1002". More suggestions?. Thank you...
01 January 2019 1,872 0 View
Hi everyone. I know that the L929 has been originated from a normal cells, however, I am a bit suspicious about L929 cells available in ATCC whether it is normal or cancer cell line? I would...
01 January 2019 1,352 6 View
01 January 2019 7,503 3 View
I'm thinking about doing a coculture with dendritic cells and a lung cancer cell line. Does anybody recommend cell lines and co-culture protocols?
27 December 2018 9,734 2 View
I'm going to extract total protein from 15 ml of a cancer cell line 24-hour conditioned medium. I need to know how much protein I can extract from this amount of CM, and is this enough for mass...
26 December 2018 3,491 7 View
Hi! I am trying to knockdown AR protein in a prostate cancer cell-line and I have used a commercial Lentiviral shRNA particles (pLKO.1 plasmid Titre 10^6). Briefly, I seeded 20,000 cells per well...
25 December 2018 8,556 3 View
Hello everyone, I am planning to perform an experiment on breast cancer cell lines treated with type I and type II interferons to see tyrosine phosphorylation of STAT1 by western blot. Would you...
12 December 2018 6,000 1 View