How do I interpret fit statistics SEM and Modindices output?

Hi, I would need some help in understanding the SEM output. I run a latent change score analysis to test the change score of a variable of interest at two-time points (repeated measure) PLD1 and...

05 January 2021 3,367 2 View

Lipofectamine 3000 in 10 cm dish?

Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...

13 October 2020 2,926 3 View

Which is the best system to keep endocrinology samples frozen in the field, without electricity?

Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...

01 September 2020 3,766 3 View

How to combine beam and shell elements in ANSYS APDL?

Hi everyone! I have a doubt and I hope there is someone who can give me an advice. I am modeling a MEMS gyroscope for my Master thesis and I am starting with a very basic structural design. My...

20 August 2020 9,524 5 View

How to edit density and heat capacity in Aspen Plus ?

Recently I'm trying to edit a pure component mass density and heat capacity of an existing component NaNO3 but failed. Could anyone help me on this? Thanks a lot.

17 July 2020 8,800 8 View

Do you have suggestions about CROSS CULTURAL ADAPTATION of questionnaires?

Hi! I am currently translating and adaptating a questionnarire about attitudes towards inclusion, and I would like to have suggestions about specific literature in translation and cross-cultural...

23 March 2020 2,630 3 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene in a common cell line?

01 March 2020 3,206 2 View

Does CXCL8 or other chemokines induce in vitro cell proliferation?

hi everybody, i have some troubles with chemokines and cell proliferation. Different papers evidence that CXCL8 (but also CXCL12, CCL2 and other chemokines) are able to promote cancer cell...

03 February 2020 214 4 View

Anyone could suggest how to analyze calcium imaging using imagej?

Hi, we would like to analyze time sequences images of calcium imaging assay performed with Fluo4-AM in imagej. Is there any plugin that we could install to do this? Do you have any kind of...

27 January 2020 2,665 1 View

Chloroform and isopropanol RNA extractions?

I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...

30 September 2019 4,647 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View