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Questions related to Cancer Cell Line
Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.
10 February 2021 2,400 6 View
Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...
05 February 2021 3,103 3 View
I have cancer cells overexpressing GFP and compared their proliferation and viability to the wild-type cells. MTT assay shows an increased signal for the cells overexpressing GFP. However, other...
03 February 2021 3,064 6 View
I am studying the recombination of X chromosome using 16 STR markers. I have 77 pedigrees each consisting of 3 generations. I used Mendel 16 software to calculate LOD scores and recombination...
29 January 2021 551 2 View
Hi every one I want to coat the 35mm matTek dish with streptavidin, I have 2 questions: 1- can I resolve the streptavidin in water instead of PBS for coating? 2- how long is enough to coat the...
27 January 2021 3,914 0 View
I want to know the detailed procedure for drying it. I am more interested to use KOH for drying. I want the procedure from starting.
15 January 2021 3,015 7 View
Hello, I am looking for ways to induce dormancy in several cancer cell lines in vitro (MC38, 4T1, B16F10, LLC etc.). Has anybody experience with this topic? What are common ways?
12 January 2021 6,024 2 View
hello when I preserve my cancer cell lines (MCF-7, HEPG-2, HCT-116) @-80 ْc for more than one month their appearance changes and after the third passage they died or contaminated how to...
08 January 2021 5,659 8 View
Recently I have been blotting PD-L1 in lung cancer cell line , but I got different results every time even with the sample protein sample, I have been different PD-L1 antibodies but I still got...
30 December 2020 1,246 3 View
I am working with apoptosis independent cancer cell treatment and what I found online included specific drug resistant or apoptotic proteins knock out cell lines. Are there any established cancer...
25 December 2020 3,899 2 View
I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...
30 November 2020 2,157 1 View
Hi, I'd like to estimate gene and isoform expression levels from RNA-Seq data with RSEM. Can it work alone (rsem-prepare-reference and rsem-calculate-expression) or does it need to be associated...
17 November 2020 995 2 View
Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...
21 October 2020 3,285 3 View
I am trying to find the most acceptable healthy cell line to compare drug cytotoxicity against breast cancer and prostate cancer cell lines in vitro. Any assistance would be appreciated.
19 October 2020 3,894 3 View
In addition, I'd like to investigate cytokeratin expression in same breast cancer cell lines but I can't decide to use which cytkokeratin?
18 October 2020 6,011 6 View
Hi everyone, does anyone have experience with performing tail vein injection with the breast cancer cell line BT549? I am looking for some advice on what the metastasis burden looks like, what...
15 October 2020 474 2 View
Hi, I have prepared chitosan nanoparticles and drug loaded chitosan nanoparticles through ionic gelation. I got a very compact pellet. I tried to dissolve them in simple deionized water, PBS...
15 October 2020 1,035 3 View
Hi everyone, I am looking for ways in which it will save me time and reduce human errors while analyzing my qPCR data. I want to eliminate or reduce steps in Excel for example, calculating delta...
01 October 2020 5,428 1 View
I have been trying to amplify a sequence of 2.2Kb by reverse transcription PCR from the HepG2 cell line. The band size I get is below 2Kb (close to 1.5Kb). I performed touchdown PCR and I added...
30 September 2020 9,697 5 View
We are doing a resistant cancer cell line through multiple exposures to a certain chemotherapeutic drug. Considering that the cell morphology had also changed, how would you know if the...
18 September 2020 979 4 View
Hi everyone, I'm doing MTT assay to test the cytotoxicity of 4 candidates on the BT-474 breast cancer cell line which rarely becomes confluent. started with 3K/well, 5K/well, 6.5K/well left the...
13 September 2020 3,344 8 View
How can I know the expression level of common tumor targeting receptors on cancer cell lines? For example the cell lines and receptors I mentioned in the question. Thanks a lot.
01 September 2020 5,600 3 View
I have a big question in my mind while doing washing and processing tissue culture and PBMC's. I saw my coullege she used 10X PBS for washing but normally 1X PBS. Is there any effect on cells or...
25 August 2020 3,876 3 View
An implant was designed and implanted on a sawbone. The implant and bone were meshed all together. As you can see in the picture attached the blue colour is the implant and green the cancellous...
12 August 2020 9,118 9 View