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Questions related to Cancer Cell Line
I am using anti-fibronectin antibodies in western blotting experiments, and in one breast cancer cell line, I found ~230kDa bands, but in other cell line of this cancer, I found bands of ~35kDa...
03 July 2020 10,179 3 View
How can i know the expression of VEGFR on certain cell line ? any recommendation !
25 June 2020 6,444 4 View
Recently, I had a below comment from a journal that asked me to do a spheroid cuture without hydrogels or matrigels. As far as I know these materials are necessary, so I tried to find the new...
02 June 2020 425 4 View
Short implants are designed for use in atrophic jaws. However, the use of short implants in a wide range should be considered. Classic 2D FEA studies by Stig Hansson (1999, 2000 and 2003) showed...
31 May 2020 9,677 4 View
Hello everybody, I'm looking for a tumor cell line (B16 or LLS) expressing OVA protein on the cell surface that I can use with OT I & OT II T cells. Does anyone have them so can share with...
22 May 2020 6,309 0 View
From total number of ovarian cancer cell lines present, for how many cell lines, sequencing data is available?
05 May 2020 3,923 2 View
Is it possible that an anticancer drug IC50 value for a particular cancerous cell line decreases on conjugation with an antibody??
23 April 2020 9,616 4 View
for immediately placed implants , to compare the surrounding alveolar bone changes through before and after CBCT scans , how to standardize the views for both scans for accurate measurements
08 April 2020 8,868 2 View
Morning All, Hopefully somebody can help, i'm struggling to be able to find the volume fraction of the two phases within the image uploaded. can anybody help? kind regards James
04 April 2020 8,109 0 View
We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...
26 March 2020 6,089 2 View
I have three breast cancer cell lines that are being treated with siRNA targeting a specific gene that is known to influence tumour growth. I've been told to leave them for 24hrs after treatment...
25 March 2020 2,619 3 View
Dear all, I'm relatively new to the "ATAC-seq technology", I performed an ATAC-seq experiment using different cell lines (non transformed and cancer cell lines) and recently I received the...
12 March 2020 7,699 4 View
Hi All, I've been treating several cancer cell lines and a non-cancer cell line with WST-1, a variation of the MTT or MST viability assays, to monitor cell death with my immunotherapy drug. All...
11 March 2020 2,511 10 View
I want to treat a cell line with a peptide tagged with biotin and detect the uptake and the localisation of the peptide through immunofluorescence. Which method should I use: a primary...
02 March 2020 5,229 0 View
I carried out a WGS of the mitochondria DNA in cancer and its corresponding adjacent tissues. The analysis show two significant SNPs with a very high OR of exposure to cancer. Consequently, I...
22 February 2020 4,015 2 View
Hello everyone, If I want to have several concentrations of a drug by using methanol as a solvent, how can I reduce or remove the toxicity of methanol in the solution? And is it necessary to do...
14 February 2020 7,061 3 View
Please let me know whether any body in this forum has first-hand experience growing them in nude mice. I was thinking about injecting 5 million cells in 1: 1 ratio of Matrigel and PBS. Let me know.
08 February 2020 4,850 1 View
02 February 2020 3,243 2 View
Carrying a lab based test using MCF-7 cancer cell line and humanized Amylin requires approval from ethics? And if it does which type of ethics in specific?
24 January 2020 3,522 5 View
Say 12 compounds against a panel of four cancer cell lines for a 72 hour IC50, for example. What should I expect to pay for that under contract?
21 January 2020 5,836 2 View
We want to analyse cell migration by using the ClearView Chemotaxis Assay from Incucyte. We put the cells to incubate for 24 hours and after each hour a photo was taken. At timepoint 0 the...
09 January 2020 1,309 0 View
I would like to use Flowing Software 2 for cell cycle analysis, does anyone have the steps for the same, or can anyone share a video tutorial, document or a link for the same.
03 January 2020 1,835 0 View
01 January 2020 8,371 5 View
Hi, I am planning to do MTT assay with H69 lung cancer cell line in 96-well plates. These cells grow as floating aggregates. Is there any method to do the assay without removing any liquid from...
01 January 2020 8,170 4 View