Contact experts in Cancer Cell Line to get answers
1,260 views 1,949 posts
Questions related to Cancer Cell Line
Hi, I have measured the senescence-associated B-Gal activity in three different cancer cell lines comparing a chemotherapy with a combination treatment. The combination treatment showed...
12 December 2018 7,990 2 View
Please suggest appropriate cancer cell line for Farnesyl Transferase Inhibitors
12 December 2018 7,078 0 View
I got bitten by lab mice treated with cancer cells. I wore gloves, but my fingers were bleeding.
12 December 2018 3,682 3 View
I see so many clinical researches in oncology how all these ideas come? I mean new risk factor in certain cancer or cell lines ... etc Do you think all these come by clinical practice or...
12 December 2018 3,963 0 View
I am working on generating breast cancer cell lines from breast cancer tissues obtained from surgery. The tissue needs to undergo some form of disaggregation to generate single cells. These are...
12 December 2018 5,446 3 View
I'm thinking about doing a coculture with dendritic cells and a lung cancer cell line. Does anybody recommend cell lines and co-culture protocols?
12 December 2018 837 2 View
I'm going to extract total protein from 15 ml of a cancer cell line 24-hour conditioned medium. I need to know how much protein I can extract from this amount of CM, and is this enough for mass...
12 December 2018 9,010 8 View
Hi! I am trying to knockdown AR protein in a prostate cancer cell-line and I have used a commercial Lentiviral shRNA particles (pLKO.1 plasmid Titre 10^6). Briefly, I seeded 20,000 cells per well...
12 December 2018 647 4 View
Does anyone have any good or detailed protocols on developing radiation-resistant cancer cell lines? We are planning to generate such cell lines to study the mechanisms behind radiation induced...
12 December 2018 4,162 3 View
11 December 2018 6,312 2 View
Can you please suggest some suitable oral cancer cell lines with highly aggressive property?
30 November 2018 6,686 3 View
I need to compare the UFC/ml I found in my positive control vs the treatment, by a division. So for example I got Positive control: -3 dilution: 1,25*10^7 UFC/ml Treatment: -2 dilution: 1,33*10^6...
12 November 2018 2,245 4 View
Hi, I want to isolate genomic DNA from buffy coat samples. Any suggestion about the best kit to purchase? Also, I am planning to measure telomere length through qPCR. Any suggestion about...
12 November 2018 4,420 0 View
Since developing drug resisntace (i.e. cisplatin resistant) takes long, is there a way to buy/get cisplatin resistant breast cancer cell lines through private lab or private research company?
11 November 2018 7,839 0 View
Type of mutations if exist.
11 November 2018 6,491 1 View
According to this literature ( Cytotoxic Activity and Chemical Composition of the Root Extract from the Mexican Species Linum scabrellum: Mechanism of Action of the Active Compound...
11 November 2018 3,685 0 View
11 November 2018 8,928 3 View
I have been trying to find values for TFR1 markers in MCF-7, OVCAR-3, MDA-MB-231, DU-145 cells etc?
11 November 2018 2,211 2 View
Thanks for every suggestion!
11 November 2018 8,871 0 View
I am working on cancer cell lines and want to check the effects of TSA on HDACs. Some article has suggested to treat the cell lysate with TSA and check the HDAC activity but we want to check the...
11 November 2018 1,506 1 View
Hello, I am looking for a good luciferase lentiviral vector with an mcherry tag to stably infect a mouse lung cancer cell line. Any suggestions? Thanks!
11 November 2018 6,833 1 View
ATCC catalog number is CRL-2505
11 November 2018 9,490 3 View
Hello, I extracted RNA from human cancer cell line, and I run agarose gel electrophoresis to detect any genomic DNA contaminants in my samples. Here are the conditions: 1% agarose gel in 0.5%...
11 November 2018 5,982 0 View
Please, any lab in Tanzania that is currently doing cytotoxicity tests on cancerous cell lines? Kindly let me know Regards
11 November 2018 3,129 0 View