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08 August 2018 2,016 0 View
I tested a natural product to treat breast cancer cell line. The cells was treated 24h and I used PI to dye the cells. I also used Doxorubicin as the control group. From the look, I can see that...
08 August 2018 6,100 3 View
After setting up 100 uL of 10000 cells/mL of the HCT-116 colorectal cancer cell line, we notice that when we place 40 uL of water in the pockets between wells, that our cells grow to about half...
08 August 2018 4,556 2 View
I am looking to set up an in vitro assay to characterize the resistance of various cancer cell lines to cytotoxic T-cell killing. I have several vials of negatively selected T-lymphocytes from...
08 August 2018 3,312 0 View
Hello Scientists, I am planning to culture prostate cancer cell line and it requires addition of Cholera toxin, I am trying to find a proper way of disposal of the cell culture media and the...
08 August 2018 9,347 3 View
A cancer cell line overexpressing egfr and vpac1 simulatneously.
08 August 2018 7,629 0 View
I have a set of breast cancer cell lines and will use HMEC as a control population. Since HMECs have the tendency to show unusual characteristics along with the passages so I would like to know...
08 August 2018 6,688 0 View
Hi, I trying to establish an ovarian cancer cell line resistant to the drug cisplatin. What is the best method to do so?
08 August 2018 3,844 3 View
06 August 2018 9,898 3 View
We are hoping to investigate myc inhibitors in breast cancer and would thus like to know the myc status of different breast cancer cell lines.
07 July 2018 5,596 0 View
I am working on gastric cancer at National Institute of Cancer Prevention and Research, an ICMR funded institute situated in I-7, Sector- 39, Noida, Uttar Pradesh, India. I need gastric cancer...
07 July 2018 9,993 0 View
FBS-free medium is what I am looking for. If defined growth factors are required, I can express them (if they are available in recombinant form). Artificial meat companies seem to have figured it...
07 July 2018 7,194 4 View
In particular in non-small-cell lung carcinoma (NSCLC) cell like A431 or in breast cancer cells like MDA-468? Someone can highlight top paper for this issue?
07 July 2018 8,277 3 View
In my recent experiments to determine phosphorylated H2AX protein in response to adding a radio mimetic drug, I have been observing abnormally high levels of the the phosphorylated H2AX in the...
07 July 2018 4,848 4 View
I see that many people use soft agar for colony formation assays with cancer cell lines. However, I also see many 3D culture protocols using Matrigel. I was under the impression that since...
07 July 2018 845 6 View
06 July 2018 1,480 3 View
How to identify Somatic Mutations in a cancer CELL LINE?
20 June 2018 6,669 3 View
•Which breast cancer cell line is good for metastasis study in NOD SCID mice model? •Hs-578t ? MDA-MB-231? MDA-MB-157? •Most of studies showed MDA-MB-231 cells are good for metastasis study,...
14 June 2018 5,454 3 View
I dump certain steroid treatments on my cancer cell lines followed by annexin staining. However even if i am observing under the microscope that there is significant cell death as all cells have...
09 June 2018 773 8 View
06 June 2018 3,375 3 View
Dear all, I am currently culturing a cancer cell line and I have noticed that there are a lot of floating cells when I am about to change media. I am interested in knowing whether these floating...
06 June 2018 3,131 2 View
06 June 2018 7,456 4 View
I would like to get in vitro work done to determine the IC50 of 20 compounds against a cancer cell line, HPAF-II preferably. What would be the likely cost of getting that work done and the...
06 June 2018 6,946 3 View
06 June 2018 3,768 9 View