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Questions related to Cancer Cell Line
Where can I find the main literature and the unique features of BT-549 (Breast cancer cell line)? Thank you!
03 March 2019 2,995 3 View
03 March 2019 5,636 2 View
I need some Arabiopsis thaliana gDNA and I do not have the facilities to perform an extraction and so I would like to purchase some. In the past I have ordered from AMSbio but they are having...
26 February 2019 7,977 5 View
Dear research helpers We are looking for a protein that is present in the nucleus of (each) human cells. The ideal protein is present with a few copies in the nucleus. Do you know of a protein...
14 February 2019 7,046 3 View
I am looking for a fractionation protocol for mouse adipose tissue (BAT and WAT)
07 February 2019 6,884 2 View
In particular DLD1 cancer cell line.
02 February 2019 508 1 View
I have been testing some inhibitors against CT26 cancer cell lines using Resazurin cell viability assay! I observe that higher inhibitor concentration showed % cell viability higher than slightly...
02 February 2019 5,895 3 View
I am culturing breast cancer cell line, some time I observe high density of tiny dot like structure, I am not able to find out whether its contamination or cell debris.if it is contamination then...
02 February 2019 7,095 1 View
I am beginner in mammalian cell culture, I am maintaining breast cancer cell line (t47d,mdamb231,mdamb468,mcf7). Cells were going well but suddenly i observed so much cell debris and slightly...
02 February 2019 9,922 4 View
Can anyone help: what is best way to prove or show the relationship between Caspase 7 induction and cell signalling pathway or other cellular proteins in PTEN knocked-out colorectal cancer cell...
02 February 2019 8,420 2 View
I am looking for any good/detailed protocols on developing drug-resistant cancer cell lines? Thanks in advance.
02 February 2019 2,393 2 View
By using plant extracts s pellet , how could I know its concentration and how much DMSO it needs to dissolve it in ?
28 January 2019 3,454 3 View
Hi all, I am planning to treat few cancer cell lines with recombinant IFN-g in-vitro. I am curious to know from researchers with prior experience if one needs to be careful of the IFN-g levels...
23 January 2019 5,863 2 View
I am planning to DNA promoter methylation in breast cancer tissue, formalin fixed paraffin embedded tissue and breast cancer cell lines. Which bisulfite modification kit will be most effective...
21 January 2019 8,656 2 View
I was trying to perform imaging with mixed glial cells in L-15 media. The microscope had some issue so I couldn't image them on time. I was wondering whether I can still image the cells in L-15...
16 January 2019 9,607 0 View
How it will work for adherent cells and cells in suspension? Is there any cells toxicity probability?
11 January 2019 1,242 3 View
I'm going to use G6PDH as cytoplasm marker but I haven't found any information about a good nucleus marker in YEASTS. Thanks!
09 January 2019 3,385 1 View
I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...
04 January 2019 1,905 3 View
bioinformatics
01 January 2019 9,514 0 View
01 January 2019 5,995 2 View
Dear all, I have some compounds which good activity against some cancer cell lines (such as DU145 (Human prostate Cancer), MDA MB-231 (human breast cancer), etc. Now I want to search for the...
01 January 2019 8,243 7 View
01 January 2019 9,951 4 View
01 January 2019 5,148 2 View
These three breast cancer cell lines are all germline mutations of BRCA1 gene?
01 January 2019 4,496 3 View