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Questions related to Cancer Cell Line
I carried out a WGS of the mitochondria DNA in cancer and its corresponding adjacent tissues. The analysis show two significant SNPs with a very high OR of exposure to cancer. Consequently, I...
22 February 2020 3,998 2 View
Hello everyone, If I want to have several concentrations of a drug by using methanol as a solvent, how can I reduce or remove the toxicity of methanol in the solution? And is it necessary to do...
14 February 2020 7,040 3 View
Please let me know whether any body in this forum has first-hand experience growing them in nude mice. I was thinking about injecting 5 million cells in 1: 1 ratio of Matrigel and PBS. Let me know.
08 February 2020 4,820 1 View
02 February 2020 3,228 2 View
Carrying a lab based test using MCF-7 cancer cell line and humanized Amylin requires approval from ethics? And if it does which type of ethics in specific?
24 January 2020 3,496 5 View
Say 12 compounds against a panel of four cancer cell lines for a 72 hour IC50, for example. What should I expect to pay for that under contract?
21 January 2020 5,830 2 View
I would like to use Flowing Software 2 for cell cycle analysis, does anyone have the steps for the same, or can anyone share a video tutorial, document or a link for the same.
03 January 2020 1,827 0 View
01 January 2020 8,362 5 View
Hi, I am planning to do MTT assay with H69 lung cancer cell line in 96-well plates. These cells grow as floating aggregates. Is there any method to do the assay without removing any liquid from...
01 January 2020 8,162 4 View
H1299 and A549 lung cancer cell lines
01 January 2020 9,721 3 View
My research is about the effects of anticancer properties from Mangifera indica Linn. peel extract against cancer. But as a high school student, our school lab doesn't allow cancer cell lines or...
12 December 2019 9,005 3 View
We are investigating myc inhibitors in breast cell lines and would like to relate response to cellular myc levels.
12 December 2019 4,235 2 View
I would like to do TFRN (Transcription factor regulatory network) analysis and I need DNase-seq bam files for cancer cell lines. I know the ENCODE but I need this information for other cell lines...
12 December 2019 8,007 3 View
Hi guys, Currently, we are culturing primary cancer cells. They look good at P1 to P3, but senescent quickly later passages. So, I am wondering how those commercial cancer cell lines were...
27 November 2019 1,925 3 View
I want to do a zymography in condition media from cancer cell line...I would like to know what should be a loading control for condition media specific for zymography? Whether loading control can...
14 November 2019 1,822 1 View
I want to know the different techniques used in the quantification of mitochondria fusion and fission in an A549 cell line or other cancer cell lines. Please help i am a Ph.D student researching...
11 November 2019 4,400 2 View
Can anyone suggest a normal colon cell and its availability in India?Thanks in advance
11 November 2019 3,677 3 View
Hello all, I have used HEPES in the past to maintain my cells outside of CO2 incubators for extended periods of time, however some of my recent work would best be performed if the bicarbonate...
06 November 2019 3,950 3 View
What is the most suitable protocol to extract nuclear protein from cancer cell lines?
30 October 2019 4,822 3 View
Hey everyone! Can anyone have information about cell lines where miR328 and miR451 overexpress? As I found, generally their expression is low in cancer cell lines, so any cell line where they are...
28 October 2019 5,368 3 View
I’m planning to co-culture 4 different cancer cell lines and label each cell line with different dye to differentiate them, then perform a drug cellular uptake experiment. Is there a device I can...
23 October 2019 3,277 3 View
I want to know the experimental and technical differences between the breast cancer cell lines SUM159 and MDA-MB-231 cells. I wonder, do they have efficiency in sphere-formation( Stemness)....
14 October 2019 6,040 3 View
Hello everyone! I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene...
14 October 2019 1,314 2 View
Hello All, Does any know a source, aside from CCLE, which contains RNA-seq FASTQ/BAM files of cancer cell lines. Thanks in advance.
10 October 2019 325 1 View