Contact experts in Cancer Cell Line to get answers
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Questions related to Cancer Cell Line
How can I measure exosome percent in my products? How can I understand these exosomes in my products are intact?
11 June 2022 5,317 4 View
Hi all, I am currently looking at the best method to quantify osteoblasts. According to the ASBMR guidelines (see below), we can quantify them by OsteoMetrics system, which is basically counting...
08 June 2022 7,017 0 View
I tried the serum-free media method
02 February 2022 4,329 8 View
I need to calculate fold change (and log fold change but I know how to do that) manually (in excel) using normalized alignment counts (counts per million). I don't want to use DESeq2 or another...
27 December 2021 4,186 1 View
Dear all, I want to construct a DIO (double-floxed inverse orientation) vector with two transgenes flanked by two sets of different lox sites (pls see image). Hence, after addition of Cre...
08 December 2021 5,816 0 View
Hello! Right now I am struggling to perform Western Blot with human, plasma-derived exosomes. I want to confirm the presence of typical, exosomal markers - e.g. Alix, CD63 and CD9. I know that I...
10 November 2021 721 4 View
the mice strain used is Balb/c mice not the Balb/c nude mice
25 October 2021 2,430 2 View
Hi, What cell density will be good to generate breast cancer in BALB/C mice? I am planning to use serum-free culture medium to suspend the cells for injection. Please recommend the best...
04 October 2021 1,613 3 View
In clonogenic assay, how to calculate the survival fraction? Is it possible to calculate using Image J software? or Any other formula required to calculate? Some articles donot show how to...
29 September 2021 9,774 0 View
Recently we overexpressed a targeted gene on the lentivirus vector. However, through repeated analysing, the elevation was validated on protein level by WB while not on mRNA level by qRT-PCR....
21 September 2021 1,155 6 View
I recently ordered a neomycin resistant plasmid to use for the transfection of human cancer cells. It came to my attention that it is recommended that I use G418 instead of the neomycin for the...
18 June 2021 9,213 0 View
I did an experiment with 12 mice (6–8 weeks) using 200.000 cells, but the tumour was regressed after 15 days. I did this experiment twice, but unfortunately, in both of them, tumours did not...
18 May 2021 7,678 1 View
Hi researchers, I have been doing cell culture for a few months now and for the first time, I see some contamination in the MCF-7 culture that we were maintaining. I would like to know what kind...
16 April 2021 8,314 7 View
I have put together a protocol below to crosslink the proteins that is potentially bound with my nuclear protein of interest. Please reply with suggestions and your experiences. 1. Dissolve DSP...
12 April 2021 980 0 View
Injected 10^5 cells subcutaneously in the 4th mammary fat pad. Distinct tumour is not formed rather the whole abdomen portion is getting swelled. What are the things that might have got wrong?...
24 March 2021 2,124 0 View
Dear all, Does someone know what these black EVs are in my UC preparation from a human cancer cell line (not known to be infected with any virus)? As you can see in the TEM scan, not only we...
22 March 2021 5,412 3 View
We are testing crude extracts taken from various parts of taro and comparing their cytotoxicity against prostate cancer. Question is, which of which is the best prostate cancer cell line in order...
12 March 2021 7,156 3 View
We are testing crude extracts taken from various parts of taro and comparing their efficiency against PCa carcinogenesis. We would like to know to incubate our prostate cancer cell line and does...
06 March 2021 8,516 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
02 March 2021 4,212 3 View
Hello, Chip-seq analysis the fragment size really matters, because currently from tap station, I am getting average 512bp reads which are (300-1500) a upper peak is in 1500bp, can any one suggest...
02 March 2021 1,412 2 View
When I studied the expression of Hypoxia Inducible transcription factor 1a from the whole cell lysate in western blot analysis, I got its expression. But when I isolated the proteins from cells...
01 March 2021 2,210 2 View
Hello! We are a group of students relatively new in the research world. We are currently working on our research concerning the efficiency of the chemopreventive agents present in Colocasia...
28 February 2021 5,966 5 View
Hello All, I need help with isolating nucleoli from cancer cell line, I want a pure fraction devoid of nuclear or cytoplasmic contamination. I tried several times with this protocol " Li ZF, Lam...
23 February 2021 1,559 0 View
Dear all, As we know, we can use the point estimate of relative risk to obtain the attributable fraction. But I am not very sure whether we can calculate the 95% confidence interval of...
15 February 2021 6,528 0 View