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Questions related to Cancer Cell Line
Hello, I am looking for a TF activation array to test human breast cancer cell lines? What about this "TF Activation Profiling Plate Array II Catalog Number FA-1002". More suggestions?. Thank you...
01 January 2019 1,905 0 View
Hi everyone. I know that the L929 has been originated from a normal cells, however, I am a bit suspicious about L929 cells available in ATCC whether it is normal or cancer cell line? I would...
01 January 2019 1,407 6 View
By using plant extracts s pellet , how could I know its concentration and how much DMSO it needs to dissolve it in ?
01 January 2019 7,536 3 View
I'm thinking about doing a coculture with dendritic cells and a lung cancer cell line. Does anybody recommend cell lines and co-culture protocols?
27 December 2018 9,777 2 View
I'm going to extract total protein from 15 ml of a cancer cell line 24-hour conditioned medium. I need to know how much protein I can extract from this amount of CM, and is this enough for mass...
26 December 2018 3,563 7 View
Hi! I am trying to knockdown AR protein in a prostate cancer cell-line and I have used a commercial Lentiviral shRNA particles (pLKO.1 plasmid Titre 10^6). Briefly, I seeded 20,000 cells per well...
25 December 2018 8,584 3 View
Hello everyone, I am planning to perform an experiment on breast cancer cell lines treated with type I and type II interferons to see tyrosine phosphorylation of STAT1 by western blot. Would you...
12 December 2018 6,038 1 View
Hi, I have measured the senescence-associated B-Gal activity in three different cancer cell lines comparing a chemotherapy with a combination treatment. The combination treatment showed...
12 December 2018 8,035 2 View
Please suggest appropriate cancer cell line for Farnesyl Transferase Inhibitors
12 December 2018 7,145 0 View
I got bitten by lab mice treated with cancer cells. I wore gloves, but my fingers were bleeding.
12 December 2018 3,725 3 View
I see so many clinical researches in oncology how all these ideas come? I mean new risk factor in certain cancer or cell lines ... etc Do you think all these come by clinical practice or...
12 December 2018 3,995 0 View
I am working on generating breast cancer cell lines from breast cancer tissues obtained from surgery. The tissue needs to undergo some form of disaggregation to generate single cells. These are...
12 December 2018 5,481 3 View
12 December 2018 880 2 View
12 December 2018 9,066 8 View
12 December 2018 681 4 View
Does anyone have any good or detailed protocols on developing radiation-resistant cancer cell lines? We are planning to generate such cell lines to study the mechanisms behind radiation induced...
12 December 2018 4,226 3 View
11 December 2018 6,373 2 View
Can you please suggest some suitable oral cancer cell lines with highly aggressive property?
30 November 2018 6,738 3 View
I need to compare the UFC/ml I found in my positive control vs the treatment, by a division. So for example I got Positive control: -3 dilution: 1,25*10^7 UFC/ml Treatment: -2 dilution: 1,33*10^6...
12 November 2018 2,277 4 View
Hi, I want to isolate genomic DNA from buffy coat samples. Any suggestion about the best kit to purchase? Also, I am planning to measure telomere length through qPCR. Any suggestion about...
12 November 2018 4,455 0 View
Since developing drug resisntace (i.e. cisplatin resistant) takes long, is there a way to buy/get cisplatin resistant breast cancer cell lines through private lab or private research company?
11 November 2018 7,878 0 View
Type of mutations if exist.
11 November 2018 6,524 1 View
According to this literature ( Cytotoxic Activity and Chemical Composition of the Root Extract from the Mexican Species Linum scabrellum: Mechanism of Action of the Active Compound...
11 November 2018 3,737 0 View
11 November 2018 8,962 3 View