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Questions related to Cancer Cell Line
ATCC catalog number is CRL-2505
07 November 2018 5,814 3 View
Hi!! I am interested in quantifying my gene of interest by qRT PCR and interested in looking for gene who's mRNA expressed either only in Nuclei or cytoplasm to identify the contamination of...
02 November 2018 3,677 2 View
I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...
31 October 2018 3,879 12 View
I have recently started to work with Flowing Software 2.5.1, Unfortunately I am stuck in the analysis of the data, because I can not modify the range the default axis values of the dot plot....
29 October 2018 9,513 7 View
Hello, I am trying to understand a co-culture experiment using different ratios of a cancer cell line and hematopoietic stem cell fraction in a mixed co culture. The ratios range from 1 to 1000...
12 October 2018 799 7 View
I am searching cancer cell line from rabbit source. VX2 is the only available cancer cell line of rabbit that I found. Could someone please give some information about this? Thanks in advance.
10 October 2018 9,874 2 View
I am exploring the inflammation based allergic responses in the colon cancer cell lines. I have two different cells HCT-116 and HCT-15. Is there any difference related to these?
10 October 2018 6,019 0 View
Many genetic changes can immortalized cancer cells including oncogene activation or tumor suppressor gene inactivation. For example telomerase activation is one way to immortalize cells. But what...
10 October 2018 7,864 6 View
10 October 2018 1,504 10 View
I was two colon cancer cell lines HCT-15 and HT-29 and tested cytotoxicity. The results obtained are completely different. The same compound has a cytotoxic effect on only one HTC-15 line....
10 October 2018 1,343 5 View
I am evaluating the uptake of florescent nanoparticles in a cancer cell line. After application of the nanoparticles, washing and harvesting the cells, do you think it is appropriate to fix the...
10 October 2018 3,624 6 View
09 October 2018 6,953 4 View
09 October 2018 7,137 2 View
I am trying to run QPCR on NK cell RNA using SYBR green. I have not been getting any amplification even in the 18S gene which we use as control. While the same primers and SYBR green works well on...
24 September 2018 1,324 3 View
Is there a limitation in the number of passages for the SK-BR-3 breast cancer cell line?
22 September 2018 3,499 4 View
I have a collection of natural product extracts that showed good anticancer potential. I'd like to screen against different cancer cell lines. I am open for any types of cooperation/...
11 September 2018 3,380 3 View
09 September 2018 2,712 4 View
Hi Everybody, I have some questions related to WST-1 cell proliferation assay. I did a WST-1 assay experiment with my pancreas cancer cell line in 96-well plate. First, I plated the cells in...
09 September 2018 7,735 0 View
09 September 2018 4,749 3 View
I was working on an enzyme that targets cancer cells. We are planning to encapuslate the enzyme with RBC (where the substrate of the enzyme can freely diffuse across membranes). However, I wonder...
09 September 2018 752 1 View
What kind of column to use for “the affinity stripping” of a biomarker from urine. Anyone have procedures with culture medium from cancer cell lines producing biomarkers too?
09 September 2018 3,456 4 View
Suppliers in Indonesia do not support selling breast cancer cells from Sigma Aldrich, so I am confused to find another supplier. I hope you guys, want to help me answer this question with complete...
09 September 2018 8,869 0 View
Hi All, According to ATCC, Neuro-2a is a Mus musculus brain neuroblastoma cell line. Further search totally confused me, because this cell line previously used to study neurite outgrowth,...
09 September 2018 8,010 4 View
Dear all, We would like to develop an gene modified cancer cell line as cancer vaccine. How do I evaluate the T cell function in mice or in human blood cells? Thanks
09 September 2018 7,961 0 View