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Questions related to Cancer Cell Line
Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...
21 October 2020 3,170 3 View
I am trying to find the most acceptable healthy cell line to compare drug cytotoxicity against breast cancer and prostate cancer cell lines in vitro. Any assistance would be appreciated.
19 October 2020 3,812 3 View
In addition, I'd like to investigate cytokeratin expression in same breast cancer cell lines but I can't decide to use which cytkokeratin?
18 October 2020 5,894 6 View
Hi everyone, does anyone have experience with performing tail vein injection with the breast cancer cell line BT549? I am looking for some advice on what the metastasis burden looks like, what...
15 October 2020 341 2 View
Hi, I have prepared chitosan nanoparticles and drug loaded chitosan nanoparticles through ionic gelation. I got a very compact pellet. I tried to dissolve them in simple deionized water, PBS...
15 October 2020 918 3 View
Hi everyone, I am looking for ways in which it will save me time and reduce human errors while analyzing my qPCR data. I want to eliminate or reduce steps in Excel for example, calculating delta...
01 October 2020 5,372 1 View
I have been trying to amplify a sequence of 2.2Kb by reverse transcription PCR from the HepG2 cell line. The band size I get is below 2Kb (close to 1.5Kb). I performed touchdown PCR and I added...
30 September 2020 9,606 5 View
We are doing a resistant cancer cell line through multiple exposures to a certain chemotherapeutic drug. Considering that the cell morphology had also changed, how would you know if the...
18 September 2020 918 4 View
Hi everyone, I'm doing MTT assay to test the cytotoxicity of 4 candidates on the BT-474 breast cancer cell line which rarely becomes confluent. started with 3K/well, 5K/well, 6.5K/well left the...
13 September 2020 3,144 8 View
How can I know the expression level of common tumor targeting receptors on cancer cell lines? For example the cell lines and receptors I mentioned in the question. Thanks a lot.
01 September 2020 5,435 3 View
I have a big question in my mind while doing washing and processing tissue culture and PBMC's. I saw my coullege she used 10X PBS for washing but normally 1X PBS. Is there any effect on cells or...
25 August 2020 3,825 3 View
i want a stander ic50 for plant to know about the strong of my crud extract plant in cancer cell line
25 July 2020 2,602 2 View
Hi there! I am currently writing up my MSc oncology dissertation; I have 5 cancer cell lines, 1 responds to a certain treatment, the other 4 do not. I am trying to find a statistical test which...
10 July 2020 3,845 4 View
I am using anti-fibronectin antibodies in western blotting experiments, and in one breast cancer cell line, I found ~230kDa bands, but in other cell line of this cancer, I found bands of ~35kDa...
03 July 2020 10,150 3 View
How can i know the expression of VEGFR on certain cell line ? any recommendation !
25 June 2020 6,419 4 View
Recently, I had a below comment from a journal that asked me to do a spheroid cuture without hydrogels or matrigels. As far as I know these materials are necessary, so I tried to find the new...
02 June 2020 418 4 View
Hello everybody, I'm looking for a tumor cell line (B16 or LLS) expressing OVA protein on the cell surface that I can use with OT I & OT II T cells. Does anyone have them so can share with...
22 May 2020 6,299 0 View
From total number of ovarian cancer cell lines present, for how many cell lines, sequencing data is available?
05 May 2020 3,908 2 View
Is it possible that an anticancer drug IC50 value for a particular cancerous cell line decreases on conjugation with an antibody??
23 April 2020 9,590 4 View
We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...
26 March 2020 6,064 2 View
I have three breast cancer cell lines that are being treated with siRNA targeting a specific gene that is known to influence tumour growth. I've been told to leave them for 24hrs after treatment...
25 March 2020 2,612 3 View
Dear all, I'm relatively new to the "ATAC-seq technology", I performed an ATAC-seq experiment using different cell lines (non transformed and cancer cell lines) and recently I received the...
12 March 2020 7,691 4 View
Hi All, I've been treating several cancer cell lines and a non-cancer cell line with WST-1, a variation of the MTT or MST viability assays, to monitor cell death with my immunotherapy drug. All...
11 March 2020 2,493 10 View
I want to treat a cell line with a peptide tagged with biotin and detect the uptake and the localisation of the peptide through immunofluorescence. Which method should I use: a primary...
02 March 2020 5,219 0 View