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Questions related to Cancer Cell Line
Has anyone had success in inducing senescence in H9c2 cells using hydrogen peroxide at low dosages (10uM -20uM) for 24 hours? I am battling to induce senescence in this cell line and am...
05 July 2022 9,950 3 View
How can I measure exosome percent in my products? How can I understand these exosomes in my products are intact?
11 June 2022 5,170 4 View
Hi all, I am currently looking at the best method to quantify osteoblasts. According to the ASBMR guidelines (see below), we can quantify them by OsteoMetrics system, which is basically counting...
08 June 2022 6,859 0 View
I tried the serum-free media method
02 February 2022 4,153 8 View
Hello! Right now I am struggling to perform Western Blot with human, plasma-derived exosomes. I want to confirm the presence of typical, exosomal markers - e.g. Alix, CD63 and CD9. I know that I...
10 November 2021 530 4 View
Hi, What cell density will be good to generate breast cancer in BALB/C mice? I am planning to use serum-free culture medium to suspend the cells for injection. Please recommend the best...
04 October 2021 1,493 3 View
In clonogenic assay, how to calculate the survival fraction? Is it possible to calculate using Image J software? or Any other formula required to calculate? Some articles donot show how to...
29 September 2021 9,664 0 View
Hi researchers, I have been doing cell culture for a few months now and for the first time, I see some contamination in the MCF-7 culture that we were maintaining. I would like to know what kind...
16 April 2021 8,150 7 View
Dear all, Does someone know what these black EVs are in my UC preparation from a human cancer cell line (not known to be infected with any virus)? As you can see in the TEM scan, not only we...
22 March 2021 5,216 3 View
We are testing crude extracts taken from various parts of taro and comparing their cytotoxicity against prostate cancer. Question is, which of which is the best prostate cancer cell line in order...
12 March 2021 7,022 3 View
We are testing crude extracts taken from various parts of taro and comparing their efficiency against PCa carcinogenesis. We would like to know to incubate our prostate cancer cell line and does...
06 March 2021 8,415 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
02 March 2021 3,715 3 View
When I studied the expression of Hypoxia Inducible transcription factor 1a from the whole cell lysate in western blot analysis, I got its expression. But when I isolated the proteins from cells...
01 March 2021 2,093 2 View
Hello! We are a group of students relatively new in the research world. We are currently working on our research concerning the efficiency of the chemopreventive agents present in Colocasia...
28 February 2021 5,840 5 View
Hello All, I need help with isolating nucleoli from cancer cell line, I want a pure fraction devoid of nuclear or cytoplasmic contamination. I tried several times with this protocol " Li ZF, Lam...
23 February 2021 1,423 0 View
Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.
10 February 2021 2,200 6 View
Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...
05 February 2021 2,881 3 View
I have cancer cells overexpressing GFP and compared their proliferation and viability to the wild-type cells. MTT assay shows an increased signal for the cells overexpressing GFP. However, other...
03 February 2021 3,006 6 View
I want to know the detailed procedure for drying it. I am more interested to use KOH for drying. I want the procedure from starting.
15 January 2021 2,929 7 View
Hello, I am looking for ways to induce dormancy in several cancer cell lines in vitro (MC38, 4T1, B16F10, LLC etc.). Has anybody experience with this topic? What are common ways?
12 January 2021 5,909 2 View
hello when I preserve my cancer cell lines (MCF-7, HEPG-2, HCT-116) @-80 ْc for more than one month their appearance changes and after the third passage they died or contaminated how to...
08 January 2021 5,525 8 View
Recently I have been blotting PD-L1 in lung cancer cell line , but I got different results every time even with the sample protein sample, I have been different PD-L1 antibodies but I still got...
30 December 2020 1,124 3 View
I am working with apoptosis independent cancer cell treatment and what I found online included specific drug resistant or apoptotic proteins knock out cell lines. Are there any established cancer...
25 December 2020 3,795 2 View
I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...
30 November 2020 1,987 1 View