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Questions related to Cancer Cell Line
That ocular give us two images of the part between us 180
24 May 2025 5,613 1 View
I've recently been raising primary microglia and macrophages, and the method of their M1 polarisation is pretty similar. Now I'm wondering how to make sure that they achieve similar levels of M1...
26 April 2025 8,428 0 View
I have purified my PCR product but after that I ran the gel and it showed double bands as shown in photo. what should I do. should I sent it for sequencing or not?
13 April 2025 285 4 View
Hi all, I am doing some drug tests with cortical neurons and I found some of them are covered by feather-like structure(shown as picture in blue). I also found this in my WT but much much less. I...
03 March 2025 9,230 3 View
définir des exigences spécifiques et des cas d'utilisation pour les xApps et les rApps. la conception, le codage et des tests des xApps et des rApps en utilisant les principes de réseau ouvert et...
20 February 2025 973 1 View
Robot
18 February 2025 8,899 0 View
I calibrated the qpcr machine 1 year ago and I have not used the machine for some experiment. I would like to know if I have to do another calibration, because I do not see any checklist that...
29 January 2025 8,967 1 View
Dear all, I am now working on in vitro transcription but struggled with low yield. One of my friends told me that the quality of plasmid, especially the proportion of supercoiled plasmids, has...
23 January 2025 4,248 3 View
"Hello, I am currently working on a project related to Novel Object Interaction (NOI) test in the field of neuroscience. I was wondering if anyone has experience or has worked with this test in...
18 January 2025 3,717 4 View
Hi all, I am working with Normal Human Epidermal Keratinocytes (NHEK) sourced from ATCC. I would like to know if any researchers here are also working with NHEK cells and can share insights,...
16 January 2025 2,334 3 View
Hello everyone I did a scratch assay test on human skin fibroblast cells. The test was done in a 24-well plate and I seeded 60,000 cells in each well at 10% FBS. After the cells reached...
06 January 2025 8,382 5 View
Uterine disease is an intensely studied part of dairy cattle health management as it heavily affects many commercial dairy farms and has serious economic consequences.
21 December 2024 4,349 2 View
Can anyone please provide me a detailed protocol to FACS out astrocytes and neurons from brain tissue? Also how much minimum amount of tiisue is needed to get proper count of astrocytes and neurons
15 December 2024 858 0 View
Anyone handy in LOD and LOQ calculation using ddPCR data? We have 10 fold serial dilution data of ddPCR.
05 November 2024 5,042 2 View
I'm trying to prepare a Sephadex-200 gel filtration column. I'm having issues with column packing and understanding how to equilibrate the column and what elution buffer to use. My sample is a...
27 October 2024 7,041 1 View
I am currently exploring the possibility of isolating newborn neurons using FACS and I was wondering if anyone here has experience with this approach. Is it feasible? If so, what markers or...
23 August 2024 5,089 0 View
I am performing plasmid DNA extraction using the akaline lysis method as described by a lab below (attached file). I wonder if I need to do any additional steps if I dissolve the extracted DNA...
02 August 2024 8,353 2 View
Hello, I am currently working on making a fusion protein through the tagging of an endogenous gene. I have restricted and ligated fragments such there has appeared a 3 amino acid (Alanine...
04 July 2024 8,221 1 View
I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?
22 May 2024 5,872 0 View
Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...
02 May 2024 3,133 0 View
I would like to know if Mitutoyo objectives (specifically Mitutoyo Infinity Corrected Long Working Distance Objectives) can be put into vacuum (10^-5 mbar) and without detoriating their...
12 April 2024 2,296 1 View
I prepare M9 Agar plates with 0.4% Lactose for selection purposes, but the desired bacteria grow in a weird manner covering all the surface of the petri dish without being able to form distinct...
07 March 2024 1,174 1 View
I'm looking for a simple UV-vis based protein ligand binding assay that a high school level or beginner college level student can perform independently. As such it would be best if it had step by...
01 March 2024 9,130 1 View
Hello dear colleagues. I am transfecting 10^6 293LTV cells in 6-well plates with lentiviral plasmids for virus production. I am doing calcium phosphate transfection with 5 mkg of DNA and homemade...
28 November 2023 2,498 0 View