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Questions related to Cancer Cell Line
I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?
22 May 2024 5,820 0 View
Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...
02 May 2024 3,075 0 View
I would like to know if Mitutoyo objectives (specifically Mitutoyo Infinity Corrected Long Working Distance Objectives) can be put into vacuum (10^-5 mbar) and without detoriating their...
12 April 2024 2,237 1 View
I prepare M9 Agar plates with 0.4% Lactose for selection purposes, but the desired bacteria grow in a weird manner covering all the surface of the petri dish without being able to form distinct...
07 March 2024 1,116 1 View
I'm looking for a simple UV-vis based protein ligand binding assay that a high school level or beginner college level student can perform independently. As such it would be best if it had step by...
01 March 2024 9,092 1 View
Hello dear colleagues. I am transfecting 10^6 293LTV cells in 6-well plates with lentiviral plasmids for virus production. I am doing calcium phosphate transfection with 5 mkg of DNA and homemade...
28 November 2023 2,449 0 View
What are the implications of integrating this protocol with Internet of Things (IoT) devices and services within a smart city, and how can it contribute to enhancing IoT-related applications?
25 October 2023 3,156 0 View
Does anyone know a reliable entity to develop a Patient-derived xenograft (PDX) model for hepatocellular carcinoma? and an estimated cost for that? I would appreciate it a lot if you could send...
08 October 2023 1,566 0 View
How does the tumor microenvironment influence the resistance to targeted therapies in glioblastoma?
29 August 2023 7,164 2 View
Hey all my cell culture fellows, i need your help and experience... i am trying to establish a cell line in our lab. i bought a fish cell line and i am now trying to get it startet. But it is...
22 August 2023 1,728 4 View
The package says 1 mg but the data sheet specifies "1-2 mg/mL in Tris-buffered saline". Any recommendations? I need to prepare coated flasks with 20 ug/mL. Thanks in advance!
04 July 2023 4,005 2 View
We use the Scratch wound assay with 2h interval. The first scan is usually on focus and demonstrates a normal wound. However, the next scans showed something else that has nothing to do with the...
21 June 2023 2,110 0 View
We would like to sublone several genes for expression, thus the genomic DNA (sales by ATCC) is not good enough for us (since few of these genes include introns. Any suggestions?
30 May 2023 8,355 0 View
Hello, I'd like to ask for your help because polycystronic vector cloning is not working well using 2A sequence. The final product we want to make is as follows restriction enzyme1 - Target1 -...
22 May 2023 6,350 2 View
I have a population dataset in whom the outcomes are 1. presence of coronary disease 2. Adverse cardiac events. In univariate analysis, the usual suspects such as LDL, smoking and family history...
08 May 2023 8,304 3 View
I am isolating yeast nucleus to check the level of my protein of interest in nucleus. I am checking the purity of my nucleus fraction with anti H3 antibody.Though I am getting a intense band of H3...
26 April 2023 7,366 0 View
I have synthesized AgNps using medicinal plant extract. I am facing a problem with their antioxidant activity. The antioxidant activity of the plant extract is higher whereas plant-mediated...
06 April 2023 1,571 3 View
I am trying to make an exosome that expresses my gene of interest. I transfect the hek293 with a plasmid that has my gene of interest. After transfection, I isolate the exosomes from the cell...
31 March 2023 9,736 1 View
Hi all, As stated in the header, I will like to know if anyone had any issues with growing NCI-295R cells in media containing FBS. I understand that many people prefer to grow them in Nu-serum,...
25 January 2023 8,608 1 View
Hello everyone, I faced a problem during my gene knockout experience, as is shown in (figure 3) I designed two primers for my targeted gene, then in (figure 4), I digested my targeted gene and...
09 December 2022 9,804 3 View
Hi, I am going to do lipofectamine transfection of my plasmid with GOI into HEK 293 and ShSY5Y cell lines. However, I have a question at the colony picking stage. How can this be done,...
11 November 2022 8,141 2 View
Hi all, I'm staining live neurons with Fluo-4 AM esters (Thermofisher cat# F14201) and seem to be having some issues when capturing images. With video recording with continuous excitation, there...
03 November 2022 2,577 0 View
We seed the BM-MSCs with 10% exosome-depleted FBS (Gibco) in culture and we couldn't see adherent cells after 24h. Should we wait for 48h or should we discharge all the flasks? If you give a...
11 October 2022 3,085 1 View
I have seen the evidence of using network analysis to conduct further cost-effectiveness analysis in two papers. The fractional polynomial models were involved because of nonproportional hazards....
12 July 2022 7,783 2 View