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Questions related to Cancer Cell Line
Hello you all! I am wanting to perform ATAC-Seq on adherent cells. So first step is to split them and have them on a cell suspension to count them. I have been told already a couple of times that...
11 September 2019 816 0 View
Hello, I'm trying to find the specific gene expression in various types of cancers and cancer cell lines, Is there any database in this regard?
09 September 2019 5,126 8 View
My current research is on Papillary thyroid cancer cell lines. For which I have purchased the cell line MDA T32 from ATCC. I need an exact description of the percentage of FBS and pen/strep to be...
09 September 2019 950 4 View
My PhD student is working on phytochemical-induced cell death in cancer cell lines and we need to establish if the cell death is apoptosis or necrosis etc. As our budget is dwindling, does anyone...
09 September 2019 3,035 4 View
I want to evaluate the expression of some miRNAs in cancerous cell lines, and I am looking for a normal cell line as a control. Can I use HEK 293T?
27 August 2019 6,639 2 View
Im using wild type SW620 cancer cell line and shCD25 SW620 cells. I want to show the significant difference in the cell viability between both the cell lines to the chemotherapeutic drug...
25 August 2019 9,912 5 View
Here I am trying to optimizing the growth of breast cancer cell which is ++- requiring additional growth factors (20% FBS, insulin and EGF) for its growth. After adding the required growth factors...
21 August 2019 4,657 2 View
From pulmonary extravasation assay , cancer cells with PGC-1a silenced were injected into tail of nude mice to observe the microevironment metastasis . I have question that PGC-1a can be supplied...
15 August 2019 2,546 1 View
Where as in case of MTT assay, the results were positive, but in DNA fragmentation assay, their is no fragmentation of genomic DNA can be seen. So, my question is, is the DNA fragmentation solely...
08 August 2019 8,145 7 View
08 August 2019 3,570 1 View
08 August 2019 8,092 2 View
Hello everyone, Is there anyone has experience in treating PT45P1 pancreatic cancer cell lines with Gemcitabin? I would really appreciate it very much if you let me know how much the Concentration...
08 August 2019 5,823 3 View
Can I treat balb/c mice with immunosuppressant and then use it for xenotransplant study? is it possible to do it this way? If not then why?
16 July 2019 4,852 1 View
I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots....
15 July 2019 2,845 3 View
I'm staining with Anti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate from Sigma on paraffin slides. What should I use for antigen retrieval, Tris-EDTA or sodium citrate?
15 July 2019 950 3 View
Thank you I observed that after treated malignant cells with NPs and analysis the expression level of caspase 3 , there are two action 1. the caspase3 has one band and upregulate 2. caspase3 has...
10 July 2019 6,802 1 View
I am looking for the mRNA expression of a particular gene which according to literature has dual role, tumor-suppressor and pro-oncogenic, now i want to compare the mRNA level of that gene in...
07 July 2019 777 5 View
I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...
07 July 2019 5,415 2 View
03 July 2019 6,039 2 View
We are attempting to induce hypoxic reponse in various breast cancer cells lines with the use of CoCl2 (more specifically using stock of 0.1M CoCl2 that was ordered already prepared and adding...
17 June 2019 5,460 3 View
Hi, Is there a good database for immortalized human cell lines? In particular cancer cell lines? Many times I need to look up attributes of a cell line; size, protein expression, doubling time,...
06 June 2019 8,500 6 View
We have isolated the exosome and preserve it at -20 degree freezer for about one month. Is the sample still suitable for morphology check under transmission electron microscopy or we must need to...
29 May 2019 9,400 5 View
Hi everybody, I want to start colony formation assay to test the potential citotoxicity of a mixture of compounds. I would like to test this mixture on 8505c cells by using this protocol:...
28 May 2019 4,652 3 View
We have tried conducting tube formation assay for RF/6A (Choroid Retinal Endothelial Cells obtained from Rhesus species). Passage number is 46. We have used MEM (ATCC recommended) medium...
27 May 2019 2,133 0 View