Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Tapestation vs Agarose gel following PCR reaction - what are the extra bands?

We recently have been moving away from using agarose gels and onto using the Tapestation (D1000 for PCR products). However, we have noticed that there are a lot of extra bands that show up on the...

15 February 2021 791 3 View

How to quantify how much of a fluorescent dye is entering a cell?

I'm looking to compare a few fluorescent dyes for cell permeability. I'm interested to see how much of the dye actually enters the cell, but would specifically like to quantify this data. Any...

21 January 2021 6,089 3 View

How to increase presynaptic neurotransmitter release artificially?

I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...

02 November 2020 5,645 3 View

Why does my plasmid grow when transformed into DH5a competent cells but not BL21?

I have a custom plasmid which expresses mCherry and will act as a reporter. I can transform this plasmid into DH5a and BL21. With DH5a I get a good number of singular colonies, but with BL21 I get...

02 September 2020 9,063 3 View

Does anyone uses casein-TBS buffer (1%) for blocking of fluorescence blots?

Hi there, we try to establish a multiplex western blotting method using fluorescence-conjugated sec. antibodies (anti-rabbit IgG StarBright Blue 520 and anti-mouse IgG StarBright Blue 700 from...

27 August 2020 7,787 5 View

Recommended measures to investigate change in psychological flexibility - ACT/CBS?

Hi All, I am looking for recommended valid measures/questionnaires to measure a change in psychological flexibility. To be delivered pre and post intervention. Can either measure psychological...

18 August 2020 8,187 3 View

How to interpret coefficient in regression when the independent variable is a change in %?

Good morning all, I am having some trouble interpreting my regression output. My model is LN of transaction volume = alpha + beta1_RER + control variables RER is defined as the change in the...

28 April 2019 516 3 View

How do you interpret Likert-scale answers to 15 questions, comparing two samples?

Part of my dissertation looks at a replication of the NEP study conducted in 2000. I'm attempting to compare the two (my repsonses and the responses received in 2000) and see whether my survey...

06 March 2019 4,050 6 View

Emulsion instability and wetting issue in microfluidic system ?

I am facing issue trying to prepare water in oil emulsion via microfluidic system using 190um quartz-made chip. The continuous phase is made of 8% polymer / DCM / 1%Span80 and the droplet phase...

05 February 2019 5,212 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View