Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator; see attached graph). This cell lines is not available via ATCC nor Sigma/ECACC. Do you know any source/cell bank/lab that provide the cell line? Thank you.
hi
you can used from PC3 & MDA-MB-231( good expression in mRNA and protein level )
good luck
you can use colorectal cancer cell lins SW480 and HCT116.
Generation of GPR55-Overexpressing Cell Lines
To stably overexpress GPR55, HCT116 and SW480 cells were transfected with a pcDNA3.1 construct coding for GPR55 with an N-terminal hemagglutinin tag (3xHA-GPR55), as described [17]. The transfection was performed with Lipofectamine 2000 according to the manufacturer’s protocol. Transfected cells were selected with 4 mg/mL G418 (Life Technologies) 48 h after transfection, grown to confluence, and subsequently sorted on a FACSAria (BD Biosciences, Franklin Lakes, NJ, USA) as described previously [13]. Sorted cells were maintained as HCT116-GPR55 (HCT55) and SW480-GPR55 (SW55) in their respective media, which were supplemented with 0.5 mg/mL G418. All assays involving pharmacological manipulation of GPR55 were performed after serum starvation and in the absence of serum and antibiotics
Colon cancer cell lines HCT116, SW480, SW620, HT29, and DLD-1 were obtained from Interlab Cell Line Collection (Genova, Italy),
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