I have done the same experiment 3 times. The first time I sorted my neurons based on expression of a neuronal marker using the FACS Aria II Sorter. It worked perfectly. The next two times I had to use the Cell Symphony S6 sorter due to a tight schedule. These two times, I did not get any cells after plating the posivitely sorted cells, although using the exact same protocol and marker. In addition, the Cell Symphony 6 Sorter counted on average 100,000 neurons per sample, which I definetely must see after plating...