We seed the BM-MSCs with 10% exosome-depleted FBS (Gibco) in culture and we couldn't see adherent cells after 24h. Should we wait for 48h or should we discharge all the flasks? If you give a response back, we will be grateful.
Hi Arslan, i am also working on MSCs and am preparing exosome depleted serum by ultracentrifugation. Initially i will be culturing in complete media until 80% confluency and will be replacing later on with conditioned media supplemented with 10% exosome depleted serum for 72hrs and will be proceeding for exosome isolation with the collected media. I haven't faced any issues with attachment while using exo-depleted serum. MSCs doesn't usually take more than a day to get attached. What did you exactly notice in the flasks?? are they just dead cells...?
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