Hi,
I am going to do lipofectamine transfection of my plasmid with GOI into HEK 293 and ShSY5Y cell lines. However, I have a question at the colony picking stage. How can this be done, especially if I do not have a microscope like the EVOS inside my hood? Our microscope is big and outside the hood, but I will not be able to pick colonies outside of the BSC because of sterility issues. Has anyone found a workaround to this?
Thanks and Regards,
Mathangi
could you not use your microscope outside the BSC and then while examining your culture on the microscope, take a marker/sharpie to mark the cells of interest on the under-side of your culture vessels and then bring your culture back into the BSC and add cloning cylinders aseptically on top/around the area of your marked regions?
Going suggest other people chime in here too with their thoughts/expertise.
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