Can we loss mCherry during sorting?

Veronica Aedo-Lopez @Veronica-Aedo-Lopez

13 November 2023 1 6K Report

Hi,

When I evaluate purity after sorting of mCherry+ cells, only 65% are positive for mCherry. Is that normal? Can cells loose fluorescence during the sorting? What can I do to improve the sorting? I am using YUMMER1.7 Luciferase+ barcoded, the barcode has the mCherry fluorescence. Thanks.

Rupa Chowdhury

I have also seen the same problem can't under why . Did you get any result

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