Any success in H9c2 induced senescence using hydrogen peroxide ?

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Lina Mesilmany

Hello,

it's 30 mmol/L of H2O2 for 2 hours and then you will have Beta Gal positive cells 3 days after stimulation. Here is the paper

DOI: 10.1371/journal.pone.0077034

Good Luck

Adam Figarski

Dear Lina, Thank You for interesting suggestion, please check the units value.

Vijayraghavan Seshadri

Increasing the concentration of H2O2 to 150uM for 24 hours might help! I use this concentration for Human derived fibroblasts.