Does anyone knows a reliable entity to develop a Patient-derived xenograft (PDX) model for hepatocellular carcinoma? and an estimated cost for that?

08 October 2023 0 2K Report

Does anyone know a reliable entity to develop a Patient-derived xenograft (PDX) model for hepatocellular carcinoma? and an estimated cost for that? I would appreciate it a lot if you could send their contacts to me at

[email protected]

Badges
Science topic

More Amr Amin's questions See All

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Does anyone know the ideal cell density for establishing TIB-75 xenograft in BALBc mice?

I am trying to establish TIB-75 cell line xenograft in BALBc mice.

19 January 2021 5,813 2 View

Which are the Non Small Cell Lung Cancer markers?

I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...

04 January 2021 7,986 3 View

Can we solate viable cells from frozen xenograft tissues?

I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...

14 December 2020 3,910 1 View

Which substance can I use as positive standard in MTT assay on A431 cell line (human epidermoid carcinoma)?

Hi everyone, I am planning to do an MTT assay for the cytotoxicity evaluation of several plant extracts on A431 cell line (human epidermoid carcinoma). For this I need to use a positive standard....

14 December 2020 1,470 4 View

Is there any possibility to buy specific drug resistant human and mouse tissue array from company or other research organisations?

I need some specific cancer tissue samples' slides for IHC which is resistant and sensitive to specific cancer drugs, especially for prostate, lung, and breast cancer. Is it possible to buy or...

27 November 2020 731 2 View

How can I make my Caki-2 cell line grow?

Hello everyone! I have trouble with a cell line, namely the human kidney carcinoma Caki-2 cell line. Another lab sent us a few criovials of cells, with their growing conditions, but I can't grow...

24 October 2020 2,568 3 View

Are my Small Cell Lung Cancer (SCLC) showing normal confluence and morphology?

Hello, I am new to cancer research and I am trying to optimize my small cell lung cancer cell protocol. I purchased a cell line from ATCC (H1105) and I have been growing the cells in RPMI 1640...

11 October 2020 5,670 6 View

How to calculate patient sample size in patient-derived xenograft study?

Greetings, i'm doing a patient-derived xenograft study and having difficulties in calculating the sample size for patients needed in my study which leads to the number of mice i need for the...

05 October 2020 8,397 3 View

I want to buy Human Squamous Cell Carcinoma Cell Lines for my phD research .?

i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .? One place is NCCS Pune...Can some give more suggestions..? Humble Regards

20 September 2020 3,940 3 View

I need to make animal model for TNBC.My institute lab do not have facilities for athymic/ nude or scid mice, can anyone guide to to proceed with this?

i want a xenograft MDA MB231 cell line animal model. can ER, PR and HER2 negative model be prepared by using an immunodeficient mouse model eg. BALB mice and which chemical can be used for this...

17 August 2020 7,279 3 View