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Questions related from Aishwarya Navnath Markad
I have performed LC-MS analysis for my fermented sample, in which I have got citric acid and all other organic acids in a specific range of retention time and mass spectra.I am trying to discuss...
11 September 2019 9,812 4 View
I am carrying out the solid state fermentation experiment using Aspergillus niger for citric acid production.I want to know how can I decide the amount of water to be added in my...
01 September 2019 8,364 5 View
I have formed a chelate using zinc sulphate and citric acid as chelating agent.This chelate was formed in a boric acid solution.I want to know the types of bonds which can be formed and between...
23 July 2019 9,617 2 View
I have added 1g of citric to 4 g of zinc sulphate in the 100 ml of boric acid solution.Now, I want to know the actual reaction happening between them?
20 June 2019 8,795 1 View
I have carried out the solid state fermentation of organic substrate using Aspergillus niger.I want to purify gallic acid from it.Can anyone suggest me simplest method to do it with references.
19 June 2019 8,478 3 View
I have carried out solid state fermentation of organic substrate using Aspergillus niger. Aspergillus niger produces variety of organic acids.I tried to extract my sample by filtration and...
18 June 2019 9,793 4 View
I want to do preparative HPLC for fermented sample.So, that I can inject the sample in MS for citric acid and gallic acid peaks.As I am not able to get proper peaks using LC-MS.Can anyone help me...
17 June 2019 10,006 4 View
I have carried out solid state fermentation of sample and using aspergillus niger citric acid is produced.Now,I want to extract this sample for analysis of LC-MS . So, I want to know in which...
17 June 2019 6,421 4 View
I have made chelate using organic acids such as citric acid and gallic acid.I have chelated metal nutrients such as zinc sulphate,copper sulphate,iron sulphate and manganese sulphate.On basis I...
17 June 2019 7,472 1 View
I have analyzed my sample using LC-Ms. The sample was fermented organic waste sample. Aspergillus niger was the microorganism used.
11 June 2019 9,987 4 View
I have carried out solid state fermentation of sample containing organic waste.Due to limited facilities, I could only do filtration and centrifugation for my sample.But,when I subjected this go...
26 May 2019 5,145 3 View
I have performed solid state fermentation using organic residue.I want to extract the sample for LC-MS estimation.Which solvent can be appropriate for the extraction of citric acid and gallic acid...
19 April 2019 616 3 View
I have performed solid state fermentation experiment for the production of citric acid using Aspergillus niger.I want to know the formula used to calculate the percentage yield of citric acid.I...
17 March 2019 264 4 View
I want to analyse my experimental data of field experiment in botany,which includes plant growth.I want to apply standard error and critical difference? I want to know what all parameters do I...
10 December 2018 7,352 5 View
In few papers,they have mentioned prescott salt is beneficial in solid state fermentation and in few papers they are saying its not..What is the key role of prescott salt and can it be uses...
10 November 2018 8,456 3 View
I want to study the effect of sucrose level at different levels in solid state fermentation in a tray bioreactor.There are lot of literature available on flask experiments but I am not getting...
05 November 2018 7,795 2 View
I want to set the Ph of substrate consisting of organic waste and leaf litter.The procedure which I followed was: 1.I took 5 g of sample and added 50 ml of D.W. in it then I started adding...
30 October 2018 6,999 3 View
I want to prepare 1N succinic acid for 0.1 N NaOH standardisation. I want to use it for of citric acid titration.
27 October 2018 5,214 2 View
I want to determine citric acid concentration using spectrophotometer from my fermented sample.I want to know the standard method to be followed for it and also the sample preparation and standard...
27 October 2018 555 2 View
Do we need 1 N succinic acid or 0.1 N succinic so as to standardise 0.1 N NaOH. I want to estimate citric acid from fermented broth using titration.I am trying to follow this protocol for it.Or...
26 October 2018 9,093 2 View
I want to standardize 0.1 N NaOH with oxalic acid. I want to know the concentration of oxalic acid to be used? After standardization, I want to use this for citric acid estimation. What can be...
25 October 2018 5,239 5 View
I want to know the exact concentrations of nutrients to be added in the fermentation media.I am using organic waste as a substrate using which I want to produce citric acid with the help of...
24 October 2018 7,764 8 View
I am carrying SSF using aspergillus niger..I m facing lot of problem of contamination..What can be done so as to avoid it?
18 October 2018 9,773 11 View
I have referred various standard protocols online for DNSA reagent preparation in which they have used 30 % of sodium potassium tartarate and later they have used 1 ml of 40% sodium potassium...
16 October 2018 2,292 0 View
I am performing solid state fermentation using aspergillus niger to produce citric acid. I have inoculated aspergillus niger spores in the culture media but I am also observing white growth.I want...
12 September 2018 668 5 View
I want to carry out solid state fermentation using garden waste as substrate, the microorganism which I am using is Aspergillus niger.I want to produce citric acid specifically from...
07 September 2018 8,730 3 View
I am performing seed germination study of fenugreek in controlled environment(lab).I want to use to germination chamber for the experiment. I want to know the temperature and relative humidity...
16 August 2018 8,617 2 View
I want to use it for solid state fermentation and the microrganism I am using is Aspergillus niger..I want to take it from agar plate and how can I decide the concentration and how can I go with...
15 August 2018 3,063 4 View
I want to carry out solid state fermentation using i.e. chicken, fish, meat waste as a substrate. The microorganism which I will be using is Aspergillus niger and my aim is to produce citric...
14 August 2018 3,527 3 View
I am performing solid state fermentation experiment using fruit waste, in which I need to vary particle size from 1 to 10mm. I want to know the simplest technique to achieve a particular particle...
03 August 2018 7,763 5 View
I am performing solid state fermentation experiment using fruit waste, in which I need to vary particle size from 1 to 10mm. I want to the simplest technique to measure its particle size?
03 August 2018 2,523 3 View
Please, provide me the standard protocol used to estimate citric acid using titration method from fermented sample.
25 July 2018 6,870 5 View
I want to use NaOH for the estimation of citric acid. For that I want to standardize NaOH with potassium dichromate. I want to know process by which it can standardized.
25 July 2018 5,053 2 View
I want to produce organic acids using solid state fermentation,I want to know which can be the suitable substrate and other conditions?
14 June 2018 9,794 3 View
I want to carry out chelation of citric acid and ZnSo4, Can I use boric acid to dissolve the ZnSo4 in it?
26 March 2018 8,456 2 View
I want to purify citric acid from fermented sample
21 March 2018 2,377 6 View
I am producing gluconic acid using fungus.I want to know how i can extract the gluconic acid and do its analysis?
14 December 2017 7,018 3 View
I am planning to design a bioreactor,which is tray type bioreactor.I am not able to find out exact papers related to this study.It would be great if anyone can help me with this.
25 February 2016 7,665 4 View
I am going to use agricultural and fruit waste as a substrate for production of citric acid, Will tray type bioreactor effective in getting better yield?
25 February 2016 5,388 2 View
I want to produce amino acids from vegetable waste using protease enzyme,but not getting the accurate method to do so.If someone has got methodology related to it.Please,help.
28 October 2015 5,330 4 View
Is there any other method used for phosphate and pottassium solubilisation? excluding with bacteria and fungi
12 August 2015 249 6 View
I am looking for postdoc positions in Indian institutes...
01 January 1970 9,902 2 View
I have carried out solid state fermentation and extracted the sample for citric acid and then analysed it on LC-MS. I have got a concentration of 50 ppm.Now, I want to calculate the concentration...
01 January 1970 360 2 View
I am performing solid state fermentation using food waste as a substrate, initially I am taking trails for 100 gram of substrate. I have referred few papers but I am not understanding that how...
01 January 1970 6,202 4 View
I want to do HPLC analysis of fermented sample.For which,I will need to store the sample for 2/3 days.I want to know which can be the proper method for its storage so that there is no loss of...
01 January 1970 5,409 3 View
