How to standardise NaOH with potassium dichromate?

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Is aliquoted basic fibroblast growth stable at 22 degree Celsius for how long?

Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable

15 June 2024 3,010 2 View

What could this structure be?

We were imaging platelets under SEM. We got this structure. Any recommendation on what this structure could be will be of great help. Thank you

02 May 2024 3,320 4 View

Require Biochemical (Quantitative) assay to detect Albumin and casein protein?

Looking for biochemical assays for quantitative estimation of Albumin and Casein protein.

28 April 2024 4,285 3 View

High mRNA expression versus low protein expression?

I tested gene expression by RT PCR followed by Western blotting to test protein expression. I get an inverse correlation with up-regulation at mRNA level and down-regulation at the protein level....

05 April 2024 4,679 6 View

How do I solve this?

I have been getting these long smeared lanes for very long in western blot. Every time I make fresh buffers and even I have changed the antibody brand. Nothing helped. Please provide suggestions...

05 November 2023 6,888 4 View

I used Spe1 and BamH1 restriction enzymes. Why is the 1st band smeared?

I used Spe1 and BamH1 restriction enzymes for checking presence of plasmid in EP1 300 strain. It had a 40kb insert size. I have got faint bands at 6kbp and at 2kbp. But the first band is smeared....

03 August 2023 7,901 0 View

Can control(untreated cells) tumor cells have >70% of G1 phase upon cell cycle analysis by PI?

After analyzing PI data of LN229 cells (glioma cell line), I observed that even the control (untreated) population had the following cell cycle proportion: G0/G1 : 79% S : 8% G2/M: 13% Is this a...

11 July 2023 6,642 2 View

why i am not getting a melt curve in my real time pcr?

When i started my real time pcr experiment i was using applied biosystems power up sybr green qpcr master mix. i was getting my results with a proper melt curve of my target gene at 83.5 degree...

21 May 2023 7,007 1 View

How to dissolve Sotalar HcL tablet?

It isnt dissolving in any solvent, i have tried water, methanol, DMSO and ethanol and also tried changing the temperature and pH. Can anyone suggest a suitable solvent or a methodology?

15 February 2023 3,689 5 View

Hi, how can we use chitosan as a stabilizer or an agent that will help to increase positive charge of overall nanoparticle suspension?

I need maximum concentration of chitosan to maintain the positive zeta potential of calcium nanoparticles. Please add your views....

30 January 2023 8,813 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Can we add calcium carbonate to any media as an indicator for lactic acid producing bacteria?

As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...

20 July 2024 7,504 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View