Do we need 1 N succinic acid or 0.1 N succinic so as to standardise 0.1 N NaOH. I want to estimate citric acid from fermented broth using titration.I am trying to follow this protocol for it.Or do I need to modify the protocol?

Estimation of Citric Acid:

Titrable Acidity Method:

Requirements:

1. Fermented broth.

2. 0.1 (N) NaOH.

3. 1.0(N) Succinic acid.

4. Phenolphthalin.

5. Titration set.

Procedure:

1. Standardise NaOH using 1(N) succinic acid.

2. Take in two separate 100 ml conical flasks 2 ml each of fermented broth and non-fermented medium (as control).

3. Titrate both, using phenolphthalin as in­dicator, end point is colourless to pink.

1 ml of 0.1 (N) NaOH = 7.0 mg of citric acid

From this calculate the yield of citric acid in g/100 ml as follows:

Titrable acidity:

Normality of NaOH using 1 (N) succinic acid = 0.07(N)

Volume of NaOH required = X ml

Estimation and Micro Estimation of Citric Acid | Biotechnology

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