I am performing solid state fermentation using food waste as a substrate, initially I am taking trails for 100 gram of substrate. I have referred few papers but I am not understanding that how much amount of aspergillus niger is to be added in the substrate so as to produce citric acid.
Is there any formula for calculation? If yes, please help me with this.
Role of Temperature, Tank Pressure and Saccharifying Enzyme in Aspergillus niger Citric Acid Fermentation Revealed by Multi-Scale Analysis
December 2016Journal of Bionanoscience 10(6):480-485
DOI: 10.1166/jbns.2016.1383
Yun ManDongsheng GuoDongsheng GuoXiujuan LiShow all 7 authorsHe Huang
I think that you can use Aspergillus niger in any concs of fungal spores in about 5 ml and add to 100 gms of the substrate ends, where these spores will be propagated.
Rock phosphate solubilization by Aspergillus niger grown on sugar-beet waste medium
- December 1995
- Applied Microbiology and Biotechnology 44(3):546-549
- 📷Nikolay Vassilev et al.
To avoid any microbial contamination, my recomendation is 2X10^6 A. niger spores per gram of dry support or dry substrate.
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