I have carried out solid state fermentation and extracted the sample for citric acid and then analysed it on LC-MS. I have got a concentration of 50 ppm.Now, I want to calculate the concentration for 100 grams of this sample.
The sample was diluted in 1:2 ratio before analysis.
Can anyone suggest me a appropriate formula for this and I also want to know that do I need to consider the peak area and mass during the concentration?