What can be done if peaks are not coming proper for organic acids using LC-MS?

More Aishwarya Navnath Markad's questions See All

Why am I seeing a fluctuating GCMS baseline/ baseline noise?

I am working with a 7820A GC and 5977B MS. Recently, we changed the helium tank and since then we have been seeing baseline noise in the chromatogram which is high enough to interfere with my...

11 November 2020 6,520 2 View

What can these peaks in PL corresponds to ?

In Zno (pristine and doped) kindly help in the analysis of these PL results at exc. 510 and 380

26 May 2020 7,393 7 View

What can be the reason of getting a negative slope in W-H plot for my synthesised nano material ?

I got negative slope (W-H plot) in my synthesised nanomaterial and on doping that same material , I got negative slope again but it's value decreased . What can I conclude from it .

01 May 2020 2,230 10 View

How to increase the yield of extracellular DNA in extraction process from water samples?

I am using 1% CTAB for extracellular DNA extraction, followed by isopropanol precipitation, phenol chloroform extraction, ethanol precipitation and wash. How can I increase my yield?

02 March 2020 7,947 3 View

For studying electrocatalytic behaviour of a nanocomposite what should I do?

I have prepared MgO nanocomposite in powder form . I want to check its electrocatalytic properties but I have no expertise in making electrodes as it is also not my guide's field. Is there any...

10 December 2019 10,027 3 View

What are the possible effects of the laser incidence angle on the sample(tissue)? Can it reduce scattering?

I am trying to build an optical tomography sytem and generate a SHG. I was wondering if changing my laser incidence angle on my sample can reduce scattering by a bit? Or is it best to keep the...

19 November 2019 7,829 4 View

Why absorbance is increasing on adding doped semiconductor NP?

I hve prepared a Doped Zno with diff conc. For photodegradation of a phenolic pollutant I got absorbance value higher. Is it mean that it can't be used for it or I should try something else

04 November 2019 3,506 4 View

What is the standard procedure to purify gallic acid from fermented sample?

I have carried out the solid state fermentation of organic substrate using Aspergillus niger.I want to purify gallic acid from it.Can anyone suggest me simplest method to do it with references.

18 June 2019 8,156 3 View

Which type of phenol has retention time between 10.91 minutes?

I have analyzed my sample using LC-Ms. The sample was fermented organic waste sample. Aspergillus niger was the microorganism used.

10 June 2019 9,710 4 View

What is the most accurate and proven method to estimate HbA1c mathematically?

Estimation of HbA1c based on the blood glucose values.

31 January 2019 2,992 9 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View