Recovery of citric acid was done after 3 days of incubation. Flasks were taken out from incubators. To 10g of fermented product, 100ml DW was added. After loosening the impregnated mycelium with the substrate using a glass rod, the flask containing the broth was shaken on an Orbital Shaker at 121rpm for 1hr. The broth was filtered using Whatman No.1 filter paper. The supernatant was tested for the pH and stored at 40C for citrate analysis
Dear NT,
After filtering off the cell mass, calcium hydroxide is added to precipitate calcium citrate and thus remove water and impurities. The precipitate is filtered off, and acidified using sulfuric acid yielding soluble citric acid and gypsum as by-product. Citric acid is crystallized from the solution. Stoichiometric amounts of gypsum are produced for recovering pure citric acid.
Good luck!
I do agree with Dr N Vassilev. On completion of citric acid synthesis by Ctrobacter it sd be filtered and the filtrate sd be precipitated with calcium hydroxide to form calcium citrate which is again filtered and the precipitate is reacted with sulphuric acid to back conversion into citric acid. Pjre form of citric acid can be got by evaporation.
Cf.:
B. Urbas, "Recovery of organic acids from a fermentation broth", US Patent 4,444,881, Apr. 24, 1984.
M.-A. Theoleyre, Y. Bathany, "Method for the purification of organic acids", US Patent 2010/0317891 A1, Dec. 16, 2010.
Cf.: M. Pazouki, T. Panda, "Recovery of citric acid ― a review", Bioprocess Engineering 19 (1998), 435-439.
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