I have carried out solid state fermentation of organic substrate using Aspergillus niger. Aspergillus niger produces variety of organic acids.I tried to extract my sample by filtration and centrifugation but I am not able to get a proper peak on LC-MS.In this case,which method can be proper for the extraction of citric acid and gallic acid from my sample.Please,suggest and provide references.
organic acids such as citric aci are soluble in water and must be detected . They might not be detected if they are formed as calcium salts and removed during filtration. You must be sure that the organic acids of your interest are being produced by the organism during fermentation.
Sir, I have got citric acid but I am not able to get its defined peak on LC. So, what can be done in this case?
You can isolate the product then try to detect. You can confirm it by IR spectroscopy.
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