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Questions related to SDS-PAGE
Do vacuoles in the Vero cell culture indicate senescence? and what is the other signs of senescence in Animal Cell Culture?
20 May 2025 9,724 2 View
We are analyzing amino acids using a C18 column (150 mm × 3 µm) with PITC:Triethylamine (1:1) derivatization, but this method is resulting in broad peaks and poor separation of the amino acids...
08 May 2025 6,296 4 View
If i use sterile- tissue culture plate for Bradford assay, will I get proper results?
07 May 2025 1,155 4 View
Investigation the efficiency of various methods of volatile oil extraction from Trichodesma africanum and their impact on the antioxidant and antimicrobial activities
06 May 2025 9,940 1 View
While IC50 concentrations can inhibit 50% of cells' viability, it may have confounding effects on gene expression. Therefore IC30 or IC40 would be better choices. Please discuss about it.
28 April 2025 8,106 1 View
I need a tool for protein-small molecule netwroking. Most of the available tools are for protein-protein networking.
14 April 2025 902 0 View
I'm looking for information on those specifically those found in Dendrobates, however it seems the knowledge and research isolated to that genus is limited. I have found contradictory information...
08 April 2025 2,961 2 View
Why are most drugs for humans tested on mice, despite the distant genetic relationship between the two species?
04 April 2025 9,810 1 View
Biomass sources examples: Construction woods, coconut fiber, lumber waste, elephant grass or any other that I might forgot
29 March 2025 8,823 1 View
Hello all, I have been trying to buffer exchange my antibody samples from a 20 mM Histidine, pH-6.0 buffer to 20 mM Sodium Acetate buffers ( pH 5.0, 5.3 & 5.6). After 10X buffer...
21 March 2025 517 0 View
Recently, we have conducted experiments following ISO 15216 standards for the detection of Norovirus GI and GII using lenticules. The lenticules were re-hydrated with 1 ml of PBS in accordance...
19 March 2025 7,249 0 View
The human brain contains 86 billion nerve cells, while the entire body also includes nerve cells? What is the basis for this specific distinction?
05 March 2025 5,676 1 View
I would like to be able to thermodynamically study the thermal decomposition of NiC2O4*2H2O to NiO. However, I cannot find reliable data on NiC2O4*2H2O, such as enthalpy of formation, standard...
22 February 2025 6,886 0 View
Bcz will have like table fruit or salad
18 February 2025 4,206 1 View
Hi everybody. I need to extract RNA from ~50 C. elegans eggs (after bleaching) or L1. For large quantity I usually freeze Eggs/larva in M9 in -80, grind in mortar with pestle in liquid nitrogen,...
29 January 2025 464 2 View
Hello I performed a standard ELISA with the following elements - Coating: IgG-hFc specific - Primary antibody + VEGF-biotin complex (start 10nM, 12 points, 1/2 dilution factor) - Secondary...
21 January 2025 7,625 2 View
As the title says, I'm looking for a fairly basic LIMS software to be used to keep track of biobanking, with reagent tracking as a nice additional feature, does anybody know of one? My main want...
16 January 2025 440 1 View
How can I check the exon composition of mRNA isoforms in the NCBI database? I am doing a CRISPR Cas9 with guide RNAs designed to target the second exon found in the "majority of the isoforms"...
14 January 2025 1,210 0 View
I am working on a project where I am using soy protein suspensions for High-Pressure Homogenization (HPH). For the pre-HPH preparation, I am mixing a 4% soy protein suspension in distilled water....
14 January 2025 4,331 0 View
I unmindfully added 1X TBST in place of washing buffer to the Ni-NTA column after the flowthrough during protein purification. Though I didn't allow the beads to incubate with the buffer, I...
07 January 2025 9,474 3 View
I am currently working on preparing activated carbon from a carbon precursor using KOH and NaOH as activating agents. After the impregnation process, when I dry the solution, the material forms a...
02 January 2025 9,435 3 View
In other words, the excitons formed by a hole with an energy below the bottom of the valence band and an electron with an energy above the ceiling of the conduction band. No matter direct or not...
09 December 2024 1,367 1 View
KIndly share the data format for phenotype and genotype (SSR) data
05 December 2024 1,455 0 View
National Security, Defense, Artificial Intelligence
23 November 2024 6,146 2 View