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Questions related to SDS-PAGE
Dear colleagues, I'm currently running some in-vitro transcription assays and I also want to check the quality on a denaturing gel-electrophoresis. However, though I have a sufficient...
27 July 2023 1,979 1 View
Hello Does eukaryote DNA extraction need to perform in sterile condition with sterile (TE) buffer? And what about plasmid extraction? I read somewhere that plasmid DNA extraction does not need to...
17 July 2023 4,267 10 View
The Brundtland Commission came out with the sustainable development theory to go beyond business as usual in social and environmental terms in 1987 assuming that traditional market dynamics and...
29 June 2023 4,177 0 View
What is the role of microbial carbon use efficiency in global soil carbon storage and role of carbon in microbial nutrition?
19 June 2023 7,230 0 View
We shall be immunizing rabbits with peptide and the blood so obtained post collection will have polyclonal antibodies. How shall we purify monoclonal antibodies from it? Please share the protocol...
16 June 2023 4,887 3 View
Detection of biofim formation using Scanning Electron Microscope
10 June 2023 4,184 3 View
We isolated several species of lactic acid bacteria (LAB) identified as Lactobacillus fermentum in 2012. This was preserved by preparing 30% glycerol stocks and was routinely revived to check...
04 May 2023 4,415 1 View
We are having a bit of a debate on weather or not the glass stoppers for our volumetric flasks should be greased. We find sometimes the stoppers on our larger flasks to be dry-locked sometimes...
11 April 2023 8,093 2 View
I've grown kenaf plants at four different Cd concentrations: 0, 100, 250, and 400 uM. Using SPAD, I measured the content of chlorophyll in the third leaf from the top. Even though there is a...
02 April 2023 4,493 1 View
When studying a certain protein, purifying the protein fused with His tag can obtain a high concentration of protein, but precipitation is prone to occur. We found that whether this protein is...
28 March 2023 8,854 0 View
Hi, Do anyone let me know in one 10 c/m dish, approximately how much NSC-34 cells can we get after they are confluent. thank you
23 March 2023 2,797 2 View
For a Si atom, band gap energy is around 1.1 eV which means that an electron in valence band needs at least this much energy to jump to conduction band. Also, at room temperature, typically energy...
21 March 2023 6,757 1 View
Hello, I have performed some recombineering protocols and realised that the chances of my plasmid being in a multimeric state are quite high. I previously designed 7 primer pairs that will...
19 March 2023 4,105 4 View
When people purify membrane protein, why they used LMNG together with CHS, or DDM together with CHS? But there is no CHS when using GDN?
16 March 2023 5,497 0 View
I collected ISF from the mouse hippocampus using a CMA probe for 25 hours, varying the collection speed. I aimed to measure Tau levels and used an ELISA kit, but no Tau proteins were detected. I...
14 February 2023 8,614 0 View
What happen to density of states of electron across metal insulator transition.
09 February 2023 4,298 5 View
I'm trying to optimize how I plan out experiments in a way that is reproducible and as hassle-free as possible. Any tips?
04 February 2023 5,923 2 View
I have a pair of primer sequences and doubt the band size it shows on the gel. Now I want to calculate the band size with the sequence I have. The problem is I tried to blast the sequence in the...
14 January 2023 2,516 1 View
Hello coleagues! I´m planning some experiments for blaKPC cloning in pCR-Blunt II TOPO. The aim of these experiments is to proof that MIC of certain antibiotics in the parental strain are due to...
27 December 2022 8,584 0 View
Hi, I'm trying to wash the gel-filtered platelets that give me supernatant and pellet, and then store them at -80 for a day. How can I do that without causing activation of the samples or platelet...
21 December 2022 9,690 3 View
I have several criovials with adipose steam cells. I thawed the frozen cells and cultured them with DMEM and 5% platelet lysate on Corning T75 flasks, but they won't attach nor grow on the flask....
30 November 2022 7,515 1 View
I am trying to culture human preadipocytes. After receiving a cryopreserved vial of preadipocytes, I cultured the cells in DMEF/F-12 media containing 10% FBS, 1% ultra-glutamine, and primocin....
24 November 2022 6,956 1 View
any help will be highly appreciated
14 October 2022 1,225 1 View
We made a DSC analysis for fish mince (Control, sukroz sorbitol added, fish protein hydrolysate added groups) for protein denaturation ratio during 6 freeze-thaw cycle. Experiment temperature...
11 October 2022 5,778 2 View