Contact experts in SDS-PAGE to get answers
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Questions related to SDS-PAGE
Does anyone have a suggestion on how to identify/see peptides on SDS-Page gels? I'm using the correct gel, but Coomassie just isn't strong enough for me to see the band.
21 March 2021 6,431 5 View
Dear researchers, My research is to determine which peptides pose antiviral activity. I used Acrylamide: Bis-Acrylamide Ratio 13:1 as this is ideal for Peptide separation and Color Marker...
19 March 2021 4,068 4 View
SDS Gel doesn't solidifies even if: - APS was made freshly - TEMED is not expired - Increased Acrylamide concentration Kindly help me if anyone have encountered similar kind of problem.
18 March 2021 8,023 8 View
I have an excel file of differentially expressed genes that includes the logFC and the logCPM. These are a prior student's data so I did not create them and I do not need all of the genes, I only...
17 March 2021 1,677 2 View
Have been finding these weird-looking objects in my culture dishes lately. There are several strange things here, but I am most concerned with the spore-like structure near the center of the field...
16 March 2021 9,121 11 View
Hi My protein of interest is only expressed in E. Coli insoluble fraction. So, I tried to extract them adding 6 M guanidine to the insoluble pellet. By rotating the mixture of pellet and solution...
12 March 2021 1,521 3 View
I have obtained these results from an SDS-PAGE. I am not sure how to analyse these results. What is the analysis that can be made and how do I determine the purity of the protein?
08 March 2021 8,812 11 View
I am trying to Induce a construct (1.3kb) from Drosophila in the pGEX-4T1 vector in BL21 cells with IPTG. IPTG induction was done in 3hr grown culture and induce for 3 to 4 hr at 37*C. But there...
06 March 2021 2,509 4 View
Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...
04 March 2021 8,507 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
03 March 2021 6,593 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
02 March 2021 3,021 3 View
Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...
26 February 2021 7,936 3 View
I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?
23 February 2021 420 2 View
I have performed SDS-PAGE on a cell lysate (25ug) prepared with 6X LB and fresh BME followed by boiling for 10 min at 95°C, but still I see signal for different epitopes (all should be around...
17 February 2021 3,051 9 View
I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...
16 February 2021 3,264 3 View
Hi everyone, I’m wondering if you could help I’ve ran a Tricine SDS-PAGE to help visualise a 2kDa glycopeptide produced by bacteria and there are several issues. The gel is a MINI-PROTEAN...
16 February 2021 3,585 3 View
I am using 29:1 Acrylamide/Bis-acrylamide solution to prepare 12 % resolving gel and 4% stacking gel. The pH of the resolving and stacking gel buffers are pH 8.8 and pH 6.8 respectively. after...
16 February 2021 1,250 1 View
I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...
15 February 2021 4,153 1 View
Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...
15 February 2021 9,326 6 View
Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...
12 February 2021 2,761 3 View
My area of research is synthesis of metal nanoparticles and their applications focusing on protein extraction from complex biological mixtures using SDS page gel electrophoresis technique. Please...
10 February 2021 3,045 3 View
I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...
08 February 2021 4,036 9 View
Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...
05 February 2021 5,288 6 View
We had several times the problem after the transfer: you can detect the yellow 10 kDa Band, the blue 20 kDa Band, the pink 25 kDa Band but after only the pink 75 kDa Band. 15, 37 and 50 kDa are...
03 February 2021 5,052 3 View