SDS-PAGE

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Questions related to SDS-PAGE

Can you help me connect with an American expert to collaborate on scrap copper recycling research, focusing on the UPCAST and electrical cable?

I am a research engineer at the École Mohammadia d'Ingénieurs in Rabat, Morocco, where I conduct research on copper scrap recycling. My work primarily focuses on optimizing recovery processes...

22 November 2024 2,844 3 View

Is AIC (or variants) appropriate for comparing gamlss models?

I have a dataset with two variables: X and Y. X is a treatment, and Y is a response. I want to model Y = X using a general additive model where I then choose one distribution from about 8 choices....

21 November 2024 3,669 0 View

If within a sample the drop of my samples was more than 25%, but my bias was correct, what should I do to reduce the drop?

18 November 2024 6,654 1 View

What should be done to reduce noise in HPGE detector?

I want to obtain a 241Am energy spectrum from my HPGe detector, but I'm encountering significant noise in this energy range. As a result, the spectrum I’ve generated is quite different from the...

17 November 2024 5,568 17 View

Conjugation of a secondary amine and protein?

Hello everyone, I want to make conjugate of a secondary amine and BSA protein. Are there any linkers can be used? And what is the condition of the reaction?

16 November 2024 2,230 1 View

In cole cole plot, how to measure total Capacitance ?

Dear Professor/Dr, in Nyquist plot, do we need to consider the electrode capacitance and Warburg? If in parallel, do we need to use parallel formula used in basic? Really Thank you

12 November 2024 4,088 3 View

Do I need to add an additional 'G' before my 20bp gRNA sequence during cloning (under U6) if the first nucleotide of my gRNA is already a 'G'?

Hello, according to published papers: https://www.nature.com/articles/nprot.2013.143/figures/4 It is recommended to have an extra guanine before the 20 bp gRNA sequence, for my efficient...

18 October 2024 9,740 1 View

Can someone help me understand size exclusion columns?

I have a BSA-FITC conjugate made in Sodium Bicarbonate pH 8.5 that I'd like to desalt (the excess dye) and buffer exchange (into PBS) via gravity. I equilibrated a G-25 column with PBS pH 7.4. I...

18 October 2024 5,977 2 View

Are those Strep-Tag II sepharose beads compatible w Glycine elution at pH:2.5?

Hello everyone, I am planning to perform IP with Streptactin sepharose beads from IBA.

13 October 2024 10,001 3 View

Which is the best wax pen to use during multistage, multiplex fluorescent staining of tissue sections?

Dear Histology Experts: We are about to embark on a huge project and wish to save time by using wax pens to provide an enclosed area in which to place our reagents during staining (previously we...

07 October 2024 6,119 0 View

How to calculate net cavity dispersion in a linear-cavity mode-locked fibre laser?

Let say I have a Mode-locked linear cavity fibre laser with 3m meter PM980 used for connect components within the cavity. Also, I am using a chirped fibre bragg grating (CFBG) for dispersion...

11 September 2024 3,967 1 View

HMF and sugar/sugar aclohol separation via HPLC?

Hello, can anybody recommend an analytical HPLC column, which detects and separates HMF and sugars/sugar alcohols properly? We have a RI detector. Thank you in advance. Kind regards, Heiko

10 September 2024 247 3 View

HPLC sugar analysis: Unknown peak at ~11.6 min?

Hi, our lab has been conducting HPLC to determine the monosaccharide composition of polysaccharide samples using the following method:Microwave-assisted extraction of polysaccharide from...

06 September 2024 6,565 2 View

Fluo4AM staining observed in DMSO negative control. Any suggestions to reduce the background?

I'm new to the Calcium measurements using the Fluo4AM dye. I'm working on titrating the amount of Fluo-4AM dye for measuring the Calcium release from HEK-293T cells. I used Thapsigargin as...

13 August 2024 5,490 1 View

Why did my RIPA buffer precipitate in the Bradford buffer?

Dear all, After protein extraction with a RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, 0,1% SDS, 1mM EDTA +PIC), I wanted to quantify the yieild by Bradford assay....

19 July 2024 7,860 3 View

How to start a research work? what are the steps to publish a research paper?

Kindly guide the entire Research paper publishing procedure

17 July 2024 2,856 2 View

When do you think we will be able to switch to hydrogen fuel in the energy sector?

06 July 2024 1,315 1 View

How can security-communication strategies be optimized to enhance citizen participation and trust in national security initiatives?

The research question, focuses on addressing key challenges in Ghana's security governance. Recent security incidents in Ghana have highlighted the critical importance of effectively communicating...

06 July 2024 4,034 2 View

No difference in 2-NBDG uptake between unstimulated, M1 and M2 polarized THP-1 cells?

Hi all, I am conducting experiments where I want to assess the glucose uptake (using 2-NBDG) in unstimulated, M1 and M2 macrophages. What I currently do is I seed the cells and polarize them...

05 June 2024 2,308 0 View

Different results from western blot and icc?

hi have you ever experienced of coming different results from western blot and icc? I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I...

26 May 2024 3,268 0 View

Why I have 2 bands at the expected molecular weight and why I have wide band?

hello everyone. Could you Please advise? I have 2 issues with my western blot:- 1) I have a wide/ broad band at the expected molecular weight (image 1) 2) I have 2 bands at the expected molecular...

23 May 2024 7,646 2 View

What sample size should be used and can this study have a hypothesis and statistically be analyzed?

we plan to detect a cell receptor (protein) that a certain type of virus with a certain serotype binds to it. we will use Chicken embryo liver cells (CEL) from SPF chickens and make three...

06 May 2024 1,983 1 View

Should it really matter if we use Tris in place of HEPES as a component of lysis buffer for his-tag pull downs using Ni-NTA beads?

For making a general mammalian cell lysis buffer, I have been using HEPES as a component. However, I came across a paper where they have used Tris in place of HEPES while the rest of the...

29 April 2024 6,226 2 View

Which of the following proliferation-tracing dyes perform the best?

I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to...

25 April 2024 2,688 0 View

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