Yes, the tissue culture plastic does not interfere. However, coatings such as fibronectin or adsorbed proteins from serum will react.

Hello Amal Raj R,

Yes you will. But ELISA plates are more cost-effective than sterile culture plates. Why use sterile tissue culture plates when ELISA plates can perform the same task?

ELISA plates provide a convenient and efficient platform for performing Bradford assay, allowing for accurate protein concentration measurements in a multi-well format. 

Best,

Of course, Amal Raj R .

You can use sterile tissue culture plates for Bradford assay.

However, it is recommended to carefully select the appropriate culture plate and make sure the plate is transparent.

It is best to use a blank control to correct the background absorbance before the experiment.

Using sterile tissue culture plates for Bradford protein assays is strongly discouraged as it frequently yields unreliable quantification results due to multiple technical limitations. The primary issue stems from the fundamental design differences between tissue culture plates and proper spectrophotometry plates - while tissue culture plates are manufactured with treated polystyrene surfaces optimized for cell adhesion, these surfaces often interfere with accurate absorbance measurements at 595 nm due to inherent light scattering properties and inconsistent optical clarity. The curved or round-bottom well geometry typical of cell culture plates creates variable light path lengths during measurement, introducing significant errors in absorbance readings compared to the uniform flat-bottom wells of assay-optimized microplates. Furthermore, the surface treatments that promote cell attachment in tissue culture plates may actively bind protein molecules from the solution, artificially depleting the available protein for dye binding and resulting in underestimated concentrations. Even with careful experimental controls, the protein-binding characteristics and potential leaching of manufacturing residues from tissue culture plastic can alter the Coomassie dye chemistry underlying the Bradford assay. For research requiring precise protein quantification, specialized flat-bottom microplates with non-binding surfaces and optical-grade clarity should always be used instead of repurposed cell culture plates, as these provide proper light transmission characteristics, consistent well geometry, and minimal protein adsorption. While emergency use of tissue culture plates with extensive controls might provide rough estimates, the substantial risk of skewed standard curves and inaccurate sample measurements makes this approach unsuitable for any quantitative analysis where precision matters. The small cost savings from reusing inappropriate plates rarely justifies the substantial risks to data integrity and experimental reproducibility in protein quantification work.