Do vacuoles in the Vero cell culture indicate senescence? and what is the other signs of senescence in Animal Cell Culture?
Hello Yasmine Mohsen,
Yes, vacuoles in cells indicate senescence. Cells undergoing senescence in culture often exhibit an increase in the number and size of vacuoles within their cytoplasm. This may be due to the accumulation of more cellular materials within the vacuoles.
Besides the increased number of vacuoles and increase in size, senescent cells are often larger and flatter than the non-senescent cells. Senescent cells also show disrupted nuclear envelope integrity due to a decrease in lamin B1 expression. Presence of vacuoles in Vero cells is an indicator of either infection or cellular stress. These cells are known for their susceptibility to vacuolization.
Please note that Vero cells are used for the production of a number of human viruses. These cells produce vacuoles, including large intracellular vacuoles, when infected with viruses, particularly at later stages of infection, and these vacuoles contain viral particles.
Best,
Thank you so much for the informative answers Dr. Malcolm Nobre , Can i have your contact please?
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