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Questions related to SDS-PAGE
how to get clear band....please provide details....attached the image
26 September 2021 431 3 View
I come from a Regenerative Medicine background. I have worked in an academic research institute but haven’t had the chance of using the LIMS. However, I came across an article on high-volume cell...
24 September 2021 4,519 1 View
I am purifying a viral protease of size 33 kda in E.coli BL-21 expression system. After cell lysis due to some unforeseen technical problems I was unable to purify my protein of interest on the...
22 September 2021 7,140 5 View
Hello, we are running sds-page electrophoresis gel of two purified protein. Both proteins have the same theoretical size but in the gels they migrated differently. Both have different Pi, one is...
21 September 2021 6,408 3 View
I am studying the effect of certain soil health products on plant protein expression. I am in particular interested on plant defence related proteins and pathogenesis related proteins. Could...
20 September 2021 9,200 3 View
Hello all, When performing SDS-PAGE electrophoresis under non-reducing conditions (without BME and DTT), disulfide bonds were broken. I am using a mPAGE 4-12% Bis-Tris Gel and MES buffer for the...
16 September 2021 9,844 6 View
I am searching to find out the electronic conductivity of PCBM and EDTA molecules to study comparison.. Which one is better? Whose electron energy density states are more (PCBM or EDTA)? Thank you...
16 September 2021 8,510 2 View
In band structures of PtSe2 - for k-path (K-G-M-K) ; for monolayer the valence band maximum (VBM) is fixed at Gamma point and while going to bilayer and higher layers, VBM shifts from Gamma point...
15 September 2021 8,353 2 View
I need to target PV interneurons in 1 and 2 months old mice.
10 September 2021 596 2 View
How to stop the glutaraldehyde crosslinking before analysis in FPLC. For SDS PAGE we add sample buffer after cross-linking to stop the reaction but for FPLC what can I do?
01 September 2021 566 4 View
I am working on photosynthesis mechanisms on a new alga strain that has no previous studies and want to separate a special LHC under a specific light enviornment. At first, I want to compare the...
01 September 2021 8,090 1 View
The power supply is working very well as well as current and still don't separate even the marker
29 August 2021 2,291 3 View
As you can see the bands are separated at the top but they are distorted at the bottom and there is more stain at the bottom while the positive control is at the top. when i satined the gel with...
28 August 2021 10,098 3 View
I am doing purification of a 6xHis-tagged protein and it seems that the protein is not eluted with the traditional 500mM Imidazole in my elution buffer...mostly contaminating proteins are present....
28 August 2021 9,307 9 View
My sample consists of a mixture of proteins. After size exclusion chromatography, the fraction samples were subjected to ESI MS. The SDS-PAGE gel showed the sIze exclusion fraction has many...
20 August 2021 7,291 3 View
Hello everyone We currently facing a problem with our SDS PAGE in our lab, as you can see in the attached file the migration at the end is very chaotic We're using BioRad products for years now,...
19 August 2021 491 4 View
I am trying to lysate BL21 DE3 E. coli for the purification of recombinant proteins Blo t 2 (14.5KDa). I couldnt achieve results in denaturing condition but in native conditions, when using...
13 August 2021 7,917 8 View
I do SDS PAGE at our lab and the results with BSA protein look great. I want to run it with a complex of Polymer and protein. However, everytime I do it, the complex always travels just like the...
11 August 2021 2,196 3 View
Dear colleagues, Please recommend a particular catalogue number of 4-VP for protein electrophoresis. We study storage high molecular proteins in wheat and get different results with different...
10 August 2021 7,954 3 View
After protein extraction i had my sample in to 2 (A&B) tubes and from each tube i made 1 stock and 3 dilutions I got the curve and each dilution's concentration How to measure the final...
06 August 2021 4,754 5 View
Hi, I'm stuck with membrane transferring in Western blot. To analyze the expression level of caspase 3 (cleaved, ~17 kDa), I harvested the cell then lysed by Laemmli sample buffer (6X, w/ DTT)...
27 July 2021 8,443 9 View
I want to do gc-ms on leaf wax. Is it possible to use a non-polar column? Do I need derivatization? Is there a protocol in this regard?
27 July 2021 5,362 3 View
Hello, We’ve run a few SDS page gels in the past few weeks but so far, we have not been getting much bands. We bought a PET-21-b+ plasmid and eventually transformed them. After growing it up in...
24 July 2021 7,559 3 View
Dear fellow researchers, I was wondering if different forms and stages of post-translational modifications (such as N-linked glycosylation) on a recombinant protein expressed in insect cells can...
21 July 2021 3,306 13 View