Gel Electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and... | Contact experts in Gel Electrophoresis to get answers

1,372 views 452 posts

Questions related to Gel Electrophoresis

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

04 March 2021 4,646 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

03 March 2021 5,769 5 View

How to determine the false positive sequencing result?

Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...

18 January 2021 3,492 11 View

What causes "bubbles" in DNA gel electrophoresis bands?

Hi all, I just ran a gel electrophoresis that shows a bubble shape in the bands of the ladder. No other row had this shape, and I haven't seen it before. What causes this? Is there anything that...

03 December 2020 1,582 4 View

Why does my gel electrophoresis appear to not have RNA?

i completed a gel electrophoresis and in the first well i have the expected outcome for tRNA,DNA and rRNA but 3 wells over there appears to be nothing. could contamination cause this or what other...

31 October 2020 7,622 15 View

Alternate method to verify Double Digestion results besides Gel Electrophoresis?

I have a plasmid vector that I have chosen two restriction sites on, unfortunately these two are only 45bp apart and I fear that gel electrophoresis will not be accurate enough to show if my...

20 October 2020 5,931 4 View

What causes thick extra line on gel electrophoresis images?

Hi All, I need help figuring out what is wrong with my gel images. I don't know the reason for the extra line outside the bright bands. Can anyone suggest a way to avoid that in the future?

06 October 2020 3,359 3 View

Best choice for Casein Zymography?

I am currently working on a project focused on MMP3. I need to perform casein zymography at some point, but I can't find a proper precast gel containing casein since the product is "discontinued"...

21 September 2020 7,920 3 View

How many bands should be collected for Library Prep when amplifying fungal ITS2 region?

I'm doing amplification of ITS2 region for fungal sequencing on Miseq. At times, 2 or three bands appeared on gel electrophoresis (range between 400-600 bp). Should all the appeared bands in this...

22 August 2020 3,754 3 View

How do I interpret my AGE results?

Hello! I am in need of help analyzing my results from Gel Electrophoresis. The first photo indicates that there was DNA extracted but did not go down. The second one shows that the DNA has been...

08 August 2020 6,275 3 View

Forgot to load a marker for gel electrophoresis, is there anyway I can measure my samples?

Hello! I really need help. I loaded my uncut, restriction digests, and PCR on my gel, and when it was done and I put it under the UV light, I realized that I forgot to load a ladder to measure my...

22 March 2020 4,655 7 View

How does a wrong (higher) concentration of loading buffer affect the results of a gel electrophoresis?

In case that the loading buffer (6x) was not diluted to 1x. It was a 2% agarose gel.

04 March 2020 1,567 4 View

What could be the best way of staining our DNA during gel electrophoresis using Gel Red? Post staining or pre-staining and what concentration?

We use gel red in our lab to stain our DNA for gel electrophoresis. The bands obtained are wavy and smeary sometimes. How shall we overcome this problem?

21 February 2020 3,465 3 View

Which brand of Agarorose gel electrophoresis system is worth?

Normal buffer tank and power supply is fine. No need expensive version but practicable design . and the price is second priority. Thank you in advance

15 January 2020 2,385 5 View

Why no bands in gel electrophoresis after extracting the plasmid from mini prep?

Hi all, I have been trying to do cloning recently. After lots of effort, I finally come to do the mini prep with iNTRON kit. When we shake the colony in 5 ml of LB with 5ul of ampicillin. There...

23 December 2019 882 3 View

Why does plasmid show different bands if dissolved in different buffers?

An 8.5kb plasmid prepared using QIAgen's Endo-toxin free MAXIprep and eluted at high concentration in the provided TE buffer. In this gel electrophoresis, the plasmid was either diluted in 1X...

23 November 2019 578 2 View

In vitro digestion with Cas9 and gRNA result?

I have gotten gel electrophoresis results of a sample of dna which I digested with Cas9 and gRNA to test the functionality of the gRNA. The results seem a little unclear to me. All the wells which...

22 November 2019 10,015 2 View

My PCRs and gels haven't been working for months, any tips?

My PCRs and gels had been working, and then they suddenly stopped for months. I tried making new reagents, lowering the annealing temperature, and making over 8 different master mixes with...

08 November 2019 4,724 25 View

Do I need to treat samples after a qPCR with Sybr Green before running a gel electrophoresis with Ethidium Bromide?

Hi all, I ran some qPCRs for my target genes and noticed some changes in meltcurves during treated samples vs control. I then decided to run a gel electrophoresis with Ethidium Bromide according...

08 November 2019 8,366 3 View

PBeloBAC1 digestion with HindIII , not sure it worked ?

Hello , So i digested pBeloBAC11 using HindIII ( left the eppendorfs 1 hour in the water bath for the digestion process ). After that the sample was frozen at -20 C and next day a gel...

05 November 2019 5,138 2 View

What is the best method to detect simultaneously VZV, JEV, CMV and EBV virus by single PCR assay and gel electrophoresis?

I want to develop an assay about virus studies. So I am searching a method to detect simultaneously Venizella zooster, Japanese encephalitis, Cytomegalo virus and Epstein barr virus using only...

05 November 2019 4,337 1 View

Your opinion on pulsed field electrophoresis instruments?

Hello, I am interested in running an experiment requiring pulsed field electrophoresis. The primary samples will be budding yeasts chromosomes and minor set of samples with chromosome sizes up...

31 October 2019 7,208 2 View

Are fuzzy bands on my gel caused by DNA inhibition?

Below is the gel image I got following a blue-white colony screening and colony PCR. I got the right bands for my inserts in lanes 2&3 but got only fuzzy, non-specific bands of roughly the same...

25 October 2019 3,562 8 View

In the PCR of CTX-M sub-types such as CTX-M-1, -2, -8, and -9?

In the PCR of CTX-M sub-types such as CTX-M-1, -2, -8, and -9, I observed when I run the gel electrophoresis, more than one band is seen. Is that how to interpret the band for the sub-types of CTX-M?

22 October 2019 7,276 5 View

Badges
Science method