Hi everybody. I need to extract RNA from ~50 C. elegans eggs (after bleaching) or L1. For large quantity I usually freeze Eggs/larva in M9 in -80, grind in mortar with pestle in liquid nitrogen, add Trizol and then extract using Zymo RNA-miniprep kit. For small quantity of eggs/L1 grinding in mortar with pestle is not an option. Just cycles of freeze-thaw in Trizol give very low RNA quantity, obviously due to insufficient breakage of eggshells/cuticles. Will be very happy to get a working protocol Suggestions are welcomed too.