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Questions related to PCR Analysis
Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...
07 October 2020 1,880 1 View
The tumor has p53 possitivity, but a comprensive PCR analysis showed no mutations in tp53.
31 March 2020 1,324 3 View
Dear colleagues, I recently jumped in this field and I was trying to recreate a study, using absolute qPCR quantification for comparison between the abundance of certain bacterial specie in two...
10 March 2020 8,370 3 View
Hello! I want to know if there is a better way to detect the presence of a live attenuated vaccine in animal blood samples. We use the buffy coat fraction to extract the DNA and perform a PCR...
18 June 2019 7,261 8 View
06 June 2019 8,699 10 View
Can we analyse muscle fibres that have been stored in RNALater by Mass Spec? We have samples stored in RNALater for PCR Analysis but would also like to run these samples on MS for protein...
05 May 2019 7,005 3 View
What are the best housekeeping genes for qPCR analysis of Arabidopsis root under nutrient stress and brassinosteroid hormone treatments?
03 March 2019 1,515 4 View
I have extracted DNA from rice leaves using the modified CTAB DNA isolation protocol, Nano drop reading is showing 1.8 to 2.00 at 260/280 ratio and the concentration is above 1800 nanogram per...
01 January 2019 2,780 6 View
I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...
12 December 2018 7,606 3 View
12 December 2018 5,474 0 View
12 December 2018 2,551 1 View
12 December 2018 5,145 1 View
I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...
12 December 2018 6,443 1 View
10 December 2018 4,692 3 View
Our study comprises of PCR analysis of DNA isolated from fecal samples at two time points (at the baseline and after intervention). We have carried out PCR and have obtained the Ct values....
26 November 2018 1,872 7 View
I have tried increasing the no. of cycles as well as checked the annealing temp but still i get a faint bands wherein i m suppose to get bright ones. even it appears same in case of housekeeping...
24 October 2018 1,611 2 View
10 October 2018 8,726 2 View
I am trying to do my first real time pcr analysis for flowering gene expression. Does primers used in routine pcr different than primers used in real time pcr? What are the criteria for designing...
03 August 2018 2,014 3 View
Hello everyone I have a semantic problem about gene loci: If a gene is deleted from a certain genome, does the gene locus still exist? For clarification: After engineering an organism by deleting...
05 May 2018 8,497 0 View
There are around 40 genes have been identified which confer resistance against Bacterial leaf blight (BLB or BB) of rice. Major genes are Xa4, Xa5, Xa13 and Xa21. I want to know the annealing...
02 February 2018 9,429 0 View
I am going to do a PCR analysis of genomic DNA of Acinetobacter isolated from clinical samples. What is the most suited conventional method for DNA extration? Can any body give a link for good...
08 January 2018 8,359 2 View
I am currently attempting to conduct PCR analysis using GoTaq polymerase (GoTaq Green Master Mix, Promega) and small primer sets from IDT that are seven basepairs in length. My problem is that...
09 September 2017 1,211 3 View
How to deal with and combine the inconsistent results? I tried to take control of my sample loading & reaction set up. As a result, I got relatively consistent expression of my internal...
03 March 2017 7,811 2 View
Hi everyone, we have a problem with our Cre-expressing cell lines, which we use to analyze cell fusion between the Cre-expressing cells and breast cancer cells expressing a double reporter vector....
19 January 2017 4,850 1 View