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Questions related to SDS-PAGE
What are the tools available to automatically detect image manipulation in research manuscript?
28 September 2025 2,069 0 View
I am looking to convert the concentration of Human IFN-gamma protein from 1000 IU/ml to mg/ml. Could anyone provide guidance on the conversion process or share the resulting concentration? Your...
25 September 2025 1,223 1 View
I am preparing to conduct in vivo animal studies with Ostamatinib (Cat. No. HY-13038A), administered via intraperitoneal injection. I have identified three different dissolution protocols using...
24 September 2025 8,530 1 View
I am working with debilitated mice and exploring the protective effects of carnosic acid on the kidneys and vascular endothelium in the context of sepsis-induced acute kidney injury, specifically...
22 September 2025 4,565 1 View
I am trying to monitor calcium signals with ATP and TRPv4 in endothelial cells (bEnd.3). For this i incubate my cells with 3-5uM Fluo-4AM in 1X HBSS (pH 6.8).after 30mins, i wash with HBSS twice...
05 September 2025 932 1 View
Dear Dr. Miwa Kubo, good morning! I would like to delve deeper into the purification methods of Ptycopetalum olacoides (marapuama Brazil) to obtain clerodane diterpenes. My name is Eduardo...
20 August 2025 2,532 0 View
I’m working with a phosphorus-containing polycarbonate (PC) flame retardant that has a DOPO-derived polyphosphonate structure. It offers intrinsic flame retardancy and thermal stability. My goal...
30 July 2025 2,849 3 View
I always produce large cell pgcc when I do cell transfection. Is there any way to avoid the production of pgcc during transfection? [Questions are selected from MedChemExpress (MCE) customer...
11 July 2025 7,692 1 View
One of the arguments against the above extension is that of added complexity , more wires at every stage etc. Could introducing SCR's (Silicon controlled rectifiers) help in realizing an analogue...
05 July 2025 1,410 1 View
I need to maintain the pH of a yeast minimal medium (initial pH: 4.3; ammonium sulfate, glucose and YNB) at pH 7 for at least 30 h post-inoculation, without using buffers. Is there a way to...
30 June 2025 6,541 0 View
I'm working with MCE Protein A/G magnetic beads (HY-K0202) where both antibody and antigen are currently bound. My goal is to recover only the antigen fraction while keeping the antibody coupled...
27 June 2025 7,550 4 View
I’m doing calcium staining assay, but the staining efficiency is pretty low and the background is really high. I’ve seen some people add Probenecid (from MedChemExpress)—what exactly does it do?...
27 June 2025 5,256 1 View
La intervención centrada en la persona tiene como finalidad promover la dignidad, los derechos y la autonomía del individuo. Se pretender abordar una intervención que conste de un equilibrio...
26 June 2025 7,771 0 View
In the laboratory, some lymphocyte cultures contain droplets whose nature and origin have yet to be identified. These droplets have no impact on cell vability or proliferation. Have other teams...
25 June 2025 5,586 1 View
Fecal samples collected from wild animals. preserved in potassium dichromate and stored at 4 degrees centigrade. DNA extraction is needed for host identification and helminth identification.
14 June 2025 2,363 1 View
I AM SCREENING A NANOBODY LIBRARY AND THE EXPRESSION IS UNDER GAL PROMOTER , THE INDUCTION IS DONE UNDER GAL PROMOTER AT 25 DEGREE C THE REPORTED EXPRESSION LEVEL IS 15% OF THE WHOLE POPULATION...
09 June 2025 1,080 0 View
Working on fine tuning mRNA isolation out of CTCs from blood. Tackling RNA degradation during the lysis and isolation step and we are considering RNase inhibitors to use in our reagents. Does...
09 June 2025 6,935 1 View
Just out of curiosity What is the per unit electricity rate for household usage in your country? Please mention your country name.For reference, in Pakistan, the electricity rates vary based on...
01 June 2025 2,300 5 View
I'm working on preparing antibacterial masterbatches for polyester filament. However, incorporating zinc oxide increases the FPV value, which negatively affects elongation and tensile strength. To...
30 May 2025 820 1 View
While using Ficoll and Histopaque, I am unable to obtain the expected four-layer separation; instead, I only observe blood cells, Ficoll, and a fat layer. Simple centrifugation and resuspension...
27 May 2025 9,956 2 View
I am trying to introduce a calcium measuring dye into 4dpf zebrafish larvae without making use of microinjections. Can anyone help with a different procedure to do this? Thank you.
27 May 2025 4,646 2 View
Does anyone have a good protocol for GABA immuno, i have tried incubated with 0.6% triton in 10% donkey serum, and primary antibody in 2 overnights, however background is high ,and signal is to...
22 May 2025 9,441 0 View
i am culturing human monocyte from pbmc after that sorting with Pan monocyte kit. i add 50ng/ml of MCSF and after 5 days add IL13, IL4 for 24 hr. to polarized them. After that i harvest and...
21 May 2025 2,200 2 View
Our histology lab uses tonsil, hoping eosinophils are present. They rarely are, so I'd like to know what other histo labs are using, please.
21 May 2025 6,971 1 View