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Questions related to SDS-PAGE
I have in stock collagen from rat tail that was purchased from Sigma but I am not sure how to prepare it for use in platelet protocols. The usual concentration is 10 ug/mL
24 August 2022 4,368 0 View
I found a few kits by Sigma-Aldrich and want to make sure I am getting the right one for my experiment. Which kit should I be getting to study co-localization of two proteins in sub-cellular...
09 August 2022 125 1 View
I would like to know suggestions for a detection and purification tag for a 150 kDa recombinant protein that is to be expressed in insect cells. This protein would be used later for immunization...
05 August 2022 3,619 3 View
Dear Researchers, I want to do a simple enzymatic experiment to explore the hexokinase activity. Since my main objective is to check the rate of hexokinase inhibition by anticancer compounds, can...
03 August 2022 911 4 View
how can I overcome inclusion bodies formation during IPTG induction when I want to express a protein with disulfide?
23 July 2022 2,317 4 View
In general, the incidence of Chilli thrips i.e. (Scirotothrips dorsalis) is was severe during the dry period. But the incidence of new invasive Chilli Thrips (Thrips parvispinus) was very high...
15 July 2022 572 0 View
I want to use electrospinning technology to make collagen and PLGA nanofibers. I used to use DCM and DMF as the solvent to dissolve collagen, but I did not find any Nitrogen from EDS.
29 June 2022 9,192 1 View
I am performing whole cell phosphoproteomics. Following cell lysis, I have trypsinized and desalted 10mg of cell lysate, which I am using for phosphoenrichment. The enrichment is antibody based,...
27 June 2022 9,972 4 View
Hi Everyone Im trying to extract and purify bacteriocin from a new species of lactic acid bacteria. Here, I extracted the bacteriocin from the MRS media using Amberlite XAD-16 (Sigma) and eluted...
13 June 2022 1,793 0 View
I am new to metabolomics and have never used GC-MS. I am analyzing the common compounds (nothing specific) present in the cell-free supernatant of Lactobacillus, and I do not know if I can submit...
31 May 2022 6,938 3 View
The total protein content of the sample is sufficient. Although the saturated concentration of ammonium sulfate was added to 85%, less than 10% of the protein was precipitated. Would the presence...
29 May 2022 7,791 3 View
Hello I want to show spirulina proteins on the sds page gel with the freezing thawing technique. Does anyone have experience with this?
13 May 2022 132 0 View
I am running ELISAs to measure antibody levels against a Plasmodium protein across three Plasmodium species. My optimized conditions are: 1. Coating antigen concentration: 2ug/ml, coating done...
25 April 2022 9,560 8 View
I'm working on photopolymerization on Whatman No.1 chromatography paper. I printed a circle using a toner laser printer and heated it inside the oven at 180 degrees Celsius for 1 hour to create...
19 April 2022 6,920 0 View
I created a hydrophobic wax barrier by printing a circle using a laser printer and putting it under the oven at 165 degrees Celcius for 15 minutes. I tried the hydrophobic wax barrier with a few...
15 April 2022 227 1 View
Hi, I want to extract amino acids and nucleosides from samples with high salt/buffer, protein, fat and cellular debris. Amino acids and nucleosides will then be analyzed by LC-MS. Do you know how...
11 April 2022 1,281 3 View
I have two groups of cells: a control, and a treated group. The genes are from cluster markers from a cluster analysis that shows a difference between the two groups. And more than half of these...
04 April 2022 5,139 1 View
I am trying to purify a His bound protein off of a Ni-NTA resin and cannot find a protocol that says the chemical make up of the solutions used to create the gradient.
17 March 2022 356 0 View
Wanted to make acetic acid buffer with ph 5.5 for phytase enzyme assay
21 February 2022 9,484 0 View
Hi everyone we tried to express a fusion constract (4 attached gene) in pet21a plasmis and E. coli. But we couldn't see any 75 kda band in SDS-page. we tested any optimization like different OD,...
14 January 2022 6,157 1 View
I have used smotic shocked method combined with TCA perception method but I cant see any result in SDS page ... so is that any other method that is more effective and I can use it as a substituted ?
09 January 2022 3,324 0 View
Good day! I need to elute proteins from polyacrylamide gel slices after BN-PAGE electrophoresis. The porblem is that the gel slices are relatively big - all the lane fragment upper from 1000 kDa...
27 December 2021 7,532 4 View
My TA for an SDS-PAGE lab stained our gels for us and ended up with the following result. He used Coomassie blue for 30 min and then he left it overnight in an unknown destaining solution. Does...
10 December 2021 3,166 4 View
I'm using px330 plasmid, cutting it with BbsI, and using annealed oligos that are 20 bp in length. I've used RE with cutsmart buffer. Purified it used a qiagen gel purification column. For the...
10 December 2021 2,063 4 View