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Questions related to SDS-PAGE
hi have you ever experienced of coming different results from western blot and icc? I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I...
26 May 2024 3,187 0 View
hello everyone. Could you Please advise? I have 2 issues with my western blot:- 1) I have a wide/ broad band at the expected molecular weight (image 1) 2) I have 2 bands at the expected molecular...
23 May 2024 7,580 2 View
we plan to detect a cell receptor (protein) that a certain type of virus with a certain serotype binds to it. we will use Chicken embryo liver cells (CEL) from SPF chickens and make three...
06 May 2024 1,899 1 View
For making a general mammalian cell lysis buffer, I have been using HEPES as a component. However, I came across a paper where they have used Tris in place of HEPES while the rest of the...
29 April 2024 6,140 2 View
I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to...
25 April 2024 2,585 0 View
In order to view/ find the laid thrip eggs is there any methods is available, if yes, suggest me the methods.
24 April 2024 7,908 0 View
I made a material which loos like liquid, but when I do the rheology test, the G'>G" . In my opinion, I think it means it was a solid or a gel. The test parameters are like this and the result...
20 April 2024 6,616 2 View
The possibility of RNApol-DnaA has been suggested by suppression of dnaA-ts by mutaion of rpoB,the initiation of replication inhibition by rifampycin and possible coupling of sugar metabolism and...
15 April 2024 1,211 0 View
I cultured Human Umbilical Vein Endothelial Cells (HUVECs) on a plate coated with collagen type 1 (rat tail) at a concentration of 7.5ug/cm^2. The coating procedure involved dilution with cell...
02 April 2024 7,265 3 View
Why does a negative or a 0 for blank mean? Does this mean that cell viability has stopped?
28 March 2024 9,445 2 View
I'm expading MSC (Mesenchymal Stem Cell) from umbilical cord uner 37 °C with 5% CO2 in a humidified chamber. I'm testing 2 types of media, both appropriate for MSC (StemPro and NutriStem). I...
19 March 2024 4,165 1 View
hi, we are working on the puc57 plasmid that our construct designed with multiple RE sites and synthesized and cloned in it. we use fast digest re enzymes (thermofisher) to separate the parts of...
22 February 2024 191 3 View
I ran restriction digestion on my plasmid just to verify my inserts and send for sequencing. my backbone plasmid is PL6 with 9843 bp containing a 3HR (877bp) and 5HR (782). My enzymes for 3HR is...
08 February 2024 8,042 4 View
What is the difference between carbon pool and carbon stock?
01 February 2024 3,344 1 View
I'm working with cell free DNA isolation from Spent culture media
18 January 2024 7,732 1 View
I'm purifying a recombinant protein. Currently, I use HIC to purify it. The specification of my Bind and Elution buffer is according to the following concentration, EC and pH... Bind, Sodium...
16 January 2024 8,186 2 View
I have data for two seasons of tamarind genotypes, and I conducted the experiment trial using a Randomized Block Design (RBD). I need to analyze this data to estimate the variability. Can someone...
06 November 2023 6,209 4 View
Is genetic diversity within populations much larger than that genetic diversity between races and ways genetic diversity can increase in a population?
28 October 2023 6,651 3 View
cotton breeding
24 October 2023 1,939 0 View
I am currently planning to conduct ploidy analysis on plants using flow cytometry. The original protocol suggests using fresh young leaves for the analysis, but I'm facing difficulties in...
11 October 2023 5,799 1 View
Hi everyone! I performed two chemical cross-linkings with my peptide using glutaraldehyde and BS3, as cross-linkers. After that, I stained the gels with the silver staining kit (Invitrogen). In...
26 September 2023 9,349 0 View
Dear QE users, I am running epw.x in quantum espresso and getting an error attached here. Please suggest me to what to do on this. Thanks, Sujata ############################################...
12 September 2023 8,970 9 View
I need to calculate the molecular dynamics of the interaction of quercetin and isorhamnetin with neuraminidase
05 September 2023 3,134 3 View
Produced wax emulsion is mixed with acrylic binder for hydrophobic coating of papers or fabrics with good adhesion. I need some useful empirical information on the formulations of raw materials...
04 September 2023 2,415 2 View