SDS-PAGE

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Questions related to SDS-PAGE

Why did my RIPA buffer precipitate in the Bradford buffer?

Dear all, After protein extraction with a RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, 0,1% SDS, 1mM EDTA +PIC), I wanted to quantify the yieild by Bradford assay....

19 July 2024 7,801 3 View

How to start a research work? what are the steps to publish a research paper?

Kindly guide the entire Research paper publishing procedure

17 July 2024 2,815 2 View

When do you think we will be able to switch to hydrogen fuel in the energy sector?

When do you think we will be able to switch to hydrogen fuel in the energy sector?

06 July 2024 1,251 1 View

No difference in 2-NBDG uptake between unstimulated, M1 and M2 polarized THP-1 cells?

Hi all, I am conducting experiments where I want to assess the glucose uptake (using 2-NBDG) in unstimulated, M1 and M2 macrophages. What I currently do is I seed the cells and polarize them...

05 June 2024 2,239 0 View

Different results from western blot and icc?

hi have you ever experienced of coming different results from western blot and icc? I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I...

26 May 2024 3,198 0 View

Why I have 2 bands at the expected molecular weight and why I have wide band?

hello everyone. Could you Please advise? I have 2 issues with my western blot:- 1) I have a wide/ broad band at the expected molecular weight (image 1) 2) I have 2 bands at the expected molecular...

23 May 2024 7,584 2 View

What sample size should be used and can this study have a hypothesis and statistically be analyzed?

we plan to detect a cell receptor (protein) that a certain type of virus with a certain serotype binds to it. we will use Chicken embryo liver cells (CEL) from SPF chickens and make three...

06 May 2024 1,904 1 View

Should it really matter if we use Tris in place of HEPES as a component of lysis buffer for his-tag pull downs using Ni-NTA beads?

For making a general mammalian cell lysis buffer, I have been using HEPES as a component. However, I came across a paper where they have used Tris in place of HEPES while the rest of the...

29 April 2024 6,147 2 View

Which of the following proliferation-tracing dyes perform the best?

I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to...

25 April 2024 2,603 0 View

How to do Thrips Egg staining?

In order to view/ find the laid thrip eggs is there any methods is available, if yes, suggest me the methods.

24 April 2024 7,919 0 View

Material is visually liquid, but rheometer shows G'>G". Why may this be?

I made a material which loos like liquid, but when I do the rheology test, the G'>G" . In my opinion, I think it means it was a solid or a gel. The test parameters are like this and the result...

20 April 2024 6,622 2 View

Some succeeded in Isolation of E.coli RNA polymerase-DnaA protein complex?

The possibility of RNApol-DnaA has been suggested by suppression of dnaA-ts by mutaion of rpoB,the initiation of replication inhibition by rifampycin and possible coupling of sugar metabolism and...

15 April 2024 1,213 0 View

Why does my HUVEC cell culture exhibit a cloudy suspension?

I cultured Human Umbilical Vein Endothelial Cells (HUVECs) on a plate coated with collagen type 1 (rat tail) at a concentration of 7.5ug/cm^2. The coating procedure involved dilution with cell...

02 April 2024 7,269 3 View

I am measuring cell viability of OD-21 cells and my hydrogels through Alamar Blue. When i calculate to get the Blank, i sometimes get a negative?

Why does a negative or a 0 for blank mean? Does this mean that cell viability has stopped?

28 March 2024 9,454 2 View

I'm expanding MSC from umbilical cord with standard culture condition, however they are differentiating. Does anyone know what might be happening?

I'm expading MSC (Mesenchymal Stem Cell) from umbilical cord uner 37 °C with 5% CO2 in a humidified chamber.  I'm testing 2 types of media, both appropriate for MSC (StemPro and NutriStem).  I...

19 March 2024 4,174 1 View

Issue with restriction enzymes. Simple and double digestion. how to solve it?

hi, we are working on the puc57 plasmid that our construct designed with multiple RE sites and synthesized and cloned in it. we use fast digest re enzymes (thermofisher) to separate the parts of...

22 February 2024 193 3 View

Why do I get three bands after restriction digestion?

I ran restriction digestion on my plasmid just to verify my inserts and send for sequencing. my backbone plasmid is PL6 with 9843 bp containing a 3HR (877bp) and 5HR (782). My enzymes for 3HR is...

08 February 2024 8,046 4 View

What is the difference between carbon pool and carbon stock?

What is the difference between carbon pool and carbon stock?

01 February 2024 3,349 1 View

Could you help me how can I make a manuscript for optimization procedure results?

I'm working with cell free DNA isolation from Spent culture media

18 January 2024 7,735 1 View

Buffer preparation for hic chromatography method?

I'm purifying a recombinant protein. Currently, I use HIC to purify it. The specification of my Bind and Elution buffer is according to the following concentration, EC and pH... Bind, Sodium...

16 January 2024 8,189 2 View

How can we calculate PCV GCV heritability and genetic advance from pooled analysis of variance or pooled ANOVA?

I have data for two seasons of tamarind genotypes, and I conducted the experiment trial using a Randomized Block Design (RBD). I need to analyze this data to estimate the variability. Can someone...

06 November 2023 6,213 4 View

Is genetic diversity within populations much larger than that genetic diversity between races and ways genetic diversity can increase in a population?

Is genetic diversity within populations much larger than that genetic diversity between races and ways genetic diversity can increase in a population?

28 October 2023 6,657 3 View

How does cotton breeding take advantage of genetic information to improve its adaptability to different growing regions?

cotton breeding

24 October 2023 1,944 0 View

What are the key genetic markers used in cotton breeding, and how do they assist in variety development?

cotton breeding

24 October 2023 3,324 0 View

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