What is the ideal mixing time for a 4%soy protein suspension before HPH, and should I adjust the pH after mixing to improve solubility and efficiency?

14 January 2025 0 4K Report

I am working on a project where I am using soy protein suspensions for High-Pressure Homogenization (HPH). For the pre-HPH preparation, I am mixing a 4% soy protein suspension in distilled water. My main question is:

How long should I mix the soy protein suspension before performing HPH to ensure adequate hydration and dispersion, especially when I am not adjusting the pH prior to mixing?

Does the solubility of soy protein vary significantly at different pH levels (neutral vs. alkaline) in the pre-HPH stage, and should I adjust the pH after mixing the suspension to test solubility?

Are there specific recommendations or best practices for mixing time and conditions (e.g., temperature, shear force) for soy protein prior to HPH to maximize the efficiency of homogenization and improve protein functionality (e.g., solubility, emulsification)?

Any insights or references would be greatly appreciated!

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Science method

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