Recently, we have conducted experiments following ISO 15216 standards for the detection of Norovirus GI and GII using lenticules. The lenticules were re-hydrated with 1 ml of PBS in accordance with the manufacturer's instructions. In ISO 15216 the procedure was filtration of spiked samples using a positive charged membrane and with a specific buffer described in ISO and a centrifugal filter concentration device we collect an eluate of the sample before proceeding to RNA extraction. In these experiments the detection of Norovirus GI is working very well. However, the detection of the Norovirus GII is limited to direct extraction of the lenticule without filtration. In all cases where water was spiked with GII and filtered there was no detection of the virus. Additionally, in all cases the PCR was performed perfectly (according to controls).

Has something familiar been reported to you? Do you have any comments or suggestions?

Kind regards