We usually use this kit which has a qia shredder column to lyse the cell before transferring the lysate onto another column for further RNA purification. However we have run out of this qia shredder.
We need to extract the RNA urgently. In the protocol stated in kit, they mention that we can use a syringe to replace the qiashredder. but they mention 'rnase free syringe'. Has anyone tried a packaged syringe that isint labelled as 'rnase free'?
Would it be fine to just use thia syringe? Would using syringe affect quality of RNA yield and is 30G,diameter too fine in diameter?
Hi!
Syringe and needles are quite efficient for homogenization. Even if there is no mark of being RNase free, sterile needles should be fine, however you can clean them with RNase Zap and then wash with RNase free water before use.
If the pellet of your cells is big, maybe you can start to use a bigger needle and then move to the little one. In this way you will not risk to get your samples blocked in the little needle or being not efficient during the homogenization.
- Badges
- Science method