TC-2 Tissue sectioner SORVALL?

I bought this tissue sectioner SORVALL TC-2, but I do not find the manual and I would like to know if someone uses this machine and can help me to understand how it works.

01 December 2020 550 2 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

Carbon content over 100 in a charcoal sample under treatment at 1200 C?

After elemental analysis of the samples, carbon content of the charcoal was 102 (several repetitions were made, but obtained the same values), the equipment was tested with other samples of wood...

02 September 2020 1,971 2 View

Can anyone help me with my contaminated human muscle cells in culture?

I am facing a problem with cell culture for weeks now. The media of the cell culture turnes turbid (white little spots, see picture) but stays reddish/pink. First this happened after 2 weeks of...

01 September 2020 1,498 9 View

Chi-Square Goodness of Fit: Can I hypothesize an expected outcome of 0 for one variable and 100 for the other variable?

If you have three variables A, B, C and you want to test whether the hypothesis is that everyone focuses on C, can the expected outcome in a chi-square goodness of fit test then be A: 0 B:0 and C:...

28 May 2020 2,008 6 View

Does anybody have a good Cytoplasmic Membrane stainning protocol?

11 February 2020 6,922 3 View

Which antibody for mouse p300 IP?

Which is the best Antibody to perform IP of endogenous mouse p300 IP. Please provide a figure or a citation in your answer. Thank you!

24 September 2019 8,482 2 View

What are good resources on how to conduct a systematic internet search of using many unique combinations of search terms?

For an evidence synthesis my colleagues and I want to check specific internet pages and databases for relevant information. A comprehensive search would include many unique combinations of search...

05 August 2019 6,736 8 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

20 January 2019 1,933 3 View

Any body working on spectroscopic investigations on functionalised fullerenes. experimental and theoretical approach

want to contact the person working in the field

08 July 2017 1,370 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View