Hi! I am need to isolate mRNA from cells for MS analysis. I used Trizol/Chloroform RNA extraction and then one kit for poly(A) selection for the mRNA isolation but I am looking for other methods to have an mRNA as much pure as possible, without rRNA contamination, in good quantity (I want to avoid waste of material) and of course I would like to check the quality of mRNA before the analysis (Is Agarose gel enough? Is it better to perform also a Northern blot?). For my purpose the ribosomal RNA contamination is a big problem. How can I remove it without degraded the mRNA? Furthermore, I would like to know exactly the mRNA quantity before starting the MS analysis just to be sure to analyze an equal amount of mRNA from the different samples. Which is the best method?
Thanks in advance for the answer.
Hi,
you may find this link helpful
https://www.thermofisher.com/iq/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/mrna-extraction.html
Good luck
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