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Questions related to Quantitative RT-PCR
It is not compatible with MS as buffer in the mobile phase for LC, but is it OK to have in sample? The concentration is around 70mM. Thanks.
07 September 2022 6,429 0 View
Hi everyone. I observed my staining and everything was okay and decided to continue the next day only to found my hoechst staining become hazy. Do you know why? I mounted them using 1x H-1400-10...
06 September 2022 7,526 0 View
I performed a titration treatment on cells in 2 replicates and then detected an antibody in the supernatant using ELISA. I have my corrected measures and standards, sample concentrations and SD...
27 August 2022 4,264 3 View
I am looking at copy number variation from frozen skin samples. The gene of interest sometimes has two bands of positive droplets across different samples. What causes this?
14 August 2022 8,429 0 View
Hi all, I'm completely new to live cell imaging and have been trying to optimise a protocol to visualise cell contractile responses using calcein, however I've been having some issues with these...
26 July 2022 6,206 3 View
If a mutation occur in noncoding DNA does it affect phenotype.
24 July 2022 7,943 1 View
Hello all, I'm looking to calibrate the UVB dose that will induce DNA damage in a monolayer of cancer cell lines/ primary cells but will keep most of them cycling. I want to follow the DNA damage...
16 July 2022 6,494 0 View
I am trying to plot Weibull distribution using Origin. In the y-axis, I am getting percentages. In most of the publications, the Y-range is different starting from some -ve value to positive (-2...
30 May 2022 4,454 1 View
Hello, I'm looking working on a clustering analysis and would be curious if anyone has ideas about how to deal with nested categorical variables. Normally I would calculate a...
28 May 2022 3,417 2 View
I am currently using an ABCAM AGP competitive ELISA kit with a dilution factor for the samples of 40,000 which was suggested by another lab. The problem is that the blank has a lower OD than the...
11 May 2022 5,871 0 View
Are there any consortium that can ship a cDNA clone in Europe?
04 May 2022 4,326 2 View
I'm doing a yeast display experiment and have been sorting yeast cells (S. cerevisiae) with a BioRad cell sorter. In the later rounds I've started getting contaminants on the plates. Some plates...
19 April 2022 4,540 2 View
Good day, could anybody suggest what could be the reason of a additional big band need upper marker? If we exclude contamination. I am using Agilent sure select XT protocol for targeted DNA seq of...
05 April 2022 6,982 0 View
We currently have a study done with RNA-seq analysis. But the raw counts in our data show a large difference, up to 10 fold higher in the raw counts versus the lowest one. But after normalization,...
01 April 2022 3,056 5 View
I ran an assay with Cy5 probe using Bio-Rad CFX96. Why are some of the curves less intense than some of the other curves? The smaller curves are using cDNA and the larger curves are using plasmid...
01 April 2022 4,635 3 View
I'm dealing with indirect ELISA, In most cases while doing PLA analysis with 4 parameters logistic fit curve Parallelism & Linearity failures are observed.
23 February 2022 563 1 View
I am doing photodegradation experiments on Contaminants of Emerging Concern, and surprisingly I see an increase in TOC from the beginning to the end of the experiment! How this could be possible?...
22 February 2022 6,781 11 View
When preparing the reaction mixture for cDNA synthesis, I messed up while adding the template. So, I had to downscale the total reaction volume for cDNA synthesis to 10 uL. I use the Thermo...
17 February 2022 1,091 2 View
Hello, if I want to know the phenotype associated with a somatic mutation, would it be possible to compare the resultant phenotype based on the phenotype reported with the corresponding germline...
26 January 2022 5,093 0 View
I was tring to check the differential binding/association of my protein to genes using mutant S. pombe. For this, I used a Flag tagged normal strain and a Flag tagged mutant strain. I did...
12 January 2022 7,247 0 View
Stability time in fresh blood before separation into serum And is it required ice ?
05 January 2022 7,211 3 View
Hi Do you know/recommend an ideal substitute for the immersion oil for fluorescence microscopy, the Immersol 518F from Zeiss? Thank you!
11 November 2021 6,726 0 View
I performed WB with 15 ug/10 ul protein sample for 1 lane My expected band is indicated in red marker in the image. But something wrong with my band, always. Band is connected by strong dots...
25 September 2021 8,546 0 View
I am studying photocatalysis of Polystyrene nanoplastics (22 nm) with TiO2 18 nm (%80 Anatase, %20 Rutile) under UVC in DI water (15 mL solution in 20 mL vials. Concentrations: 20 ppm PS, 20 ppm...
18 September 2021 7,225 5 View