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Questions related to Quantitative RT-PCR
I have very few copper mesh to isolate my InVivo ephy implants and I'm trying to find a distributor in Europe but still not successful found one that sells small amount of it. The item reference...
14 September 2021 3,949 0 View
I am imaging cells which have endogenous GFP signal and I stained them with DAPI in mounting medium. I use confocal ZEISS LSM800. Some of the cell soma (looks like cytoplasm and processes) seems...
14 September 2021 9,393 4 View
I am looking for a lock box to store a biotoxin in my -80 freezer? Does anyone know of any that can withstand temperatures that low? Thanks}!
03 September 2021 3,390 0 View
I am experimenting degradation of polystyrene nanoparticle using TiO2 and I face higher final TOC values than initial TOC. I assume it is due to the generation of CO2 while mineralization. However...
15 August 2021 4,189 5 View
Hello everyone, I have conducted a multi-phenotype GWAS analysis. I have 5 phenotypes in total, the GWAS lambda ranged from 0.8 to 1.12, only two of them are acceptable (0.985, 1.030). Is there...
10 August 2021 6,337 0 View
I am having trouble with Nanog IHC in ESCs and iPSCs. In all of the cell lines that I am characterizing, I have measured mRNA expression of Oct4, Sox2, and Nanog, and I have successfully stained...
19 July 2021 3,503 1 View
I have cDNA samples extracted from 90+ individuals which I intend to utilise in a series of qPCR experiments to assess the influence of a specific SNP genotype/s on target gene/s expression. I...
09 July 2021 3,008 3 View
Can I use 'quantitect reverse transcription kit' storaged in refrigeration temperature for 20 days? Originally, it should be stored at -20 celsius temperature scale.
22 June 2021 6,930 3 View
I want to isolate extracellular DNA from 1L of environmental sample (lake water). Is it possible to concentrate the ext. DNA in sample using anion exchange columns ? What are important...
21 June 2021 7,663 0 View
Hi everyone. For my project I have to reduce the time for reverse transcription of mRNA. We are using High Capacity cDNA Reverse Transcription Kit from Applied Biosystems. It's recommended there...
16 June 2021 8,294 3 View
Hi folks I published a couple of things in Nature Education Knowledge and they were well received; but they stopped producing new content. Is anyone aware of similar educational outlets? I'm...
08 June 2021 1,536 0 View
Explain the unequal control and treatment group effect on choices of econometric methods
08 June 2021 6,431 5 View
Good day great scholars. Please I would appreciate help on how to install Discovery Studio and PyRx in Ubuntu. I will also like to know how I can assess/identify the path of the License Pack for...
27 May 2021 9,827 10 View
We are struggling with inconsistent settlement and early life survival with sea cucumber (sandfish, Holothuria scabra) in a semi commercial hatchery in tropical Northern Territory, Australia. We...
19 May 2021 3,345 0 View
I try to develop a gradient hplc analysis method for two drugs/ the 2nd one composed of 3 compounds, but an unknown peak appears at 8.5 min as shown in the attached photo of injected blank...
12 May 2021 4,054 3 View
Does anyone have experience growing single cell clones of MCF-10A.I have generated gene knockouts and need to pick single cell clones from these cell lines. It is challenging as these cells are...
29 April 2021 1,878 2 View
I am doing a Chip-seq experiment on one human sample and testing the DNA segment I got from Chip by qPCR first. What I found is my primer designed based on UCSC Genome Browser doesn't work very...
19 April 2021 2,440 2 View
I have been trying to find the copy number variation for the knocked in mCherry gene in U2OS cells. I am using the Thermofisher TaqMan Copy Number Assay kit with the custom probe for the mCherry...
12 April 2021 1,845 0 View
Hello friends! I have coded VUMAT subroutine and when i submitted the job through job module its showing 'Running'. But it is not displaying any results or increment in monitor option. It is...
12 April 2021 9,767 0 View
Hello everyone, Here is my situation. I have some nucleotide sequences from which amino acid sequences were predicted using Prodigal. Then an hmmsearch of a target amino acid sequence was...
05 April 2021 1,376 6 View
So I'm using this code to try and control the Keithley 2450 using a 64bit win 10 pc, im running all this in jupyternotebook. The code I'm running: " import numpy as np import pandas as...
02 April 2021 650 0 View
These are initially solitary rod like small structures floating in the media, and then they start forming aggregates. Could this be bacillus? How to deal with it.
30 March 2021 7,720 6 View
Hello, I want to work with some gene expression data that is recorded in TPM. The data has two biological replicates with their own TPM values. How should I go about using this data? is there a...
23 March 2021 8,079 3 View
In order to increase humidity to 99% I have to put a container filled with water in side (Thermo Scientific) CO2 incubator. The problem is that due to evaporation of water, water collects at...
16 March 2021 2,238 3 View