Hi all, I am looking to test relative gene expression for my gene of interest in comparison to a reference gene. I realised, belatedly, I'd need a standard curve for every plate and I am wondering if I can pool samples into a singular curve or if every sample needs its own dilution curve? I will attach pictures of hypothetical plate set-ups. I could just test it and mess around and see if pooled samples work fine, but I didn't want to reinvent the wheel if there is literature supporting either or. With the latter, I imagine that would take way too many resources, but I am unsure. Thank you for any help anyone can provide! If helpful, I am using a Bio-Rad CFX96.
- Badges
- Science method
More Del Hannay's questions See All
Similar questions and discussions