Molecular identification of number of bacteria in saliva by using RT-PCR
This technique gave us the only Cq but we need the starting bacterial level in saliva
HI Ahed Abdullah,
For a specific gene absolute quantification in an efficient qPCR, you need to dress a standard curve by using at less two dilutions (of 10-fold dilution of the known amount standard). If you don't have standards, you can make your own by making 10 fold dilution of bacterial culture and running a couple of repetitions for each dilution to have a certain repeatability to get a stable Cq for each dilution. Thus, you will have an idea of the range of Cq values corresponding to an initial bacterial load. There are many references discussing the use of qPCR for relative and absolute quantification:
Article Simple Absolute Quantification Method Correcting for Quantit...
Article Quantification of Intestinal Bacterial Populations by Real-T...
Article Comparison among the Quantification of Bacterial Pathogens b...
Article Optimizing qPCR for the Quantification of Periodontal Pathog...
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